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Microscopy

Microscopy. Light and Electron Microscopy. The First Light Microscopes. Around 1590 Zaccharias and Hans Janssen experimented with lenses in a tube, lead to first microscope. In the late 1600’s, Anton van Leeuwenhoek was the first to use a microscope. Father of Microscopy.

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Microscopy

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  1. Microscopy Light and Electron Microscopy

  2. The First Light Microscopes • Around 1590 Zaccharias and Hans Janssen experimented with lenses in a tube, lead to first microscope. • In the late 1600’s, Anton van Leeuwenhoek was the first to use a microscope. Father of Microscopy.

  3. How Light Microscopes Work • First, the objective lens gathers light from the specimen and magnifies the image • objective lenses (4X, 10X, 40X) • The ocular lens in the eyepiece magnifies and transmits the image to your eye • ocular lens is 10X • total magnification = multiply the objective lens x ocular lens. • For example: 40X (objective lense) x 10X (ocular lense) = 400X magnification

  4. Microscope Parts Eyepiece BodyTube RevolvingNosepiece Arm ObjectiveLens Stage StageClips CoarseFocus Diaphragm FineFocus Light Base

  5. The Parts of a Light Microscope • Light source: Could be a mirror, but most likely it is a bulb built into the base • Diaphragm: Adjusts the amount of light striking an object • Objective lens: Gathers light and magnifies image • Ocular lens (eyepiece): Magnifies objects and focuses light to your eye • Stage: Holds slide • Can be moved using the coarse or fine adjustment knobs to bring the object into focus • Stage clips: Hold slide in place • Base and arm: Structural support for the microscope

  6. Microscope Care • Always carry with 2 hands • Only use lens paper for cleaning • Do not force knobs • Always store covered • Keep desk clear of objects and cords

  7. Using the Microscope • Place the Slide on the Microscope • Use Stage Clips • Click Nosepiece to the lowest (shortest) setting • Look into the Eyepiece • Use the Coarse Focus

  8. Using High Power • Follow steps to focus using low power • Click the nosepiece to the longest objective • Do NOTuse the Coarse Focusing Knob • Use the Fine Focus Knob to bring the slide into focus

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