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Microscopy - PowerPoint PPT Presentation


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By Jane Horlings. Microscopy. Microscopy. Robert Hooke, circa 1700s. Electron Microscope, late 1900s. Light Microscopes. Early light microscope (UL), drawing by Hooke (LL). Light Microscopes. Dissecting (stereo) microscope (L), compound microscope (R). Light Microscopy; Plant Cells.

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Presentation Transcript
microscopy1
Microscopy

Robert Hooke, circa 1700s

Electron Microscope, late 1900s

light microscopes
Light Microscopes

Early light microscope (UL), drawing by Hooke (LL)

light microscopes1
Light Microscopes

Dissecting (stereo) microscope (L), compound microscope (R)

light microscopy plant cells
Light Microscopy; Plant Cells

Onion root tip, cell division (L), shoot tip (R)

light microscopy phase contrast
Light Microscopy, Phase Contrast

Single celled Amoeba dividing (L), green alga Micrasterias (R)

slide7

Light Microscopy

Fluorescence

Confocal

Phase contrast

limitations of electron microscopy
Limitations of Electron Microscopy
  • Works in a vacuum
  • Specimens are dead, chemically preserved; no life processes can be seen

• No color (colorized by artist on computer)

electron microscopes1
Electron Microscopes

Transmission Electron Microscope (TEM)

Scanning Electron Microscope (SEM)

scanning electron microscopy
Scanning Electron Microscopy

TEM of cilia

SEM of cilia

slide12

Bacterium

SEM

RBCs in clot

slide13

SEM

Mite (UL)

Gecko toes (LL)

Shark skin (LR)

slide14

SEM

Trypanosome

Red blood cells

Red blood cells and Trypanosoma (L), Giardia (R)

transmission electron microscopy
Transmission Electron Microscopy

TEM of cilia

SEM of cilia

slide16

TEM

Viruses (L), animal cell (R)

slide17

TEM

Animal cell (L), muscle tissue (R)

light microscopy
Light Microscopy
  • Based on light
  • Specimens can be alive; life processes can be seen

• Color; dyes may be used

parts of the microscope
Parts of the Microscope
  • Ocular lenses
  • Objective lenses
  • How to compute the magnification
use of the microscope
Use of the Microscope
  • Place slide in center
  • Adjust light, lenses, barrel
    • Put on low magnification!
    • Move stage all the way up and then back down half a turn!
use of the microscope1
Use of the Microscope
  • Look and readjust focus
  • Move to higher magnification if needed
use of the microscope2
Use of the Microscope
  • Importance of focusing with the fine adjustment!
  • Oil immersion lens
  • How to adjust the light