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0 4 8 12 24 48 (hr)

100 nM Paclitaxel . 0 4 8 12 24 48 (hr). Cleaved Caspase-9. Caspase-8. MCF-7. Figure S1a. **. *. MCF-7 . Figure S1b. Paclitaxel 100 nM. - + - + - +. Bad siRNA. - - + + - -. Scrambled siRNA.

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0 4 8 12 24 48 (hr)

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  1. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 MCF-7 Figure S1a

  2. ** * MCF-7 Figure S1b

  3. Paclitaxel 100 nM - + - + - + Bad siRNA - - + + - - Scrambled siRNA - - - - + + Bad Actin BimEL BimL IP: Bcl-2 IB: Bim BimS IP: Bcl-2 IB: Bcl-2 Bcl-2 Input IB: Bcl-2 Bcl-2 Actin MCF-7 Figure S1c

  4. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 T47D Cleaved Caspase-3 Figure S1d

  5. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 MDA-MB-468 Cleaved Caspase-3 Figure S1e

  6. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 BT20 Cleaved Caspase-3 Figure S1f

  7. Paclitaxel 100 nM 0 4 8 12 [h] Bim EL IP: Bcl-xL IB: Bim Bim L Bim S IP: Bcl-xL IB: Bcl-xL Bcl-xL MCF-7 Paclitaxel 100 nM 0 4 8 12 [h] Bim EL IP: Mcl-1 IB: Bim Bim L Bim S IP: Mcl-1 IB: Mcl-1 Mcl-1 Figure S2a

  8. Pre-IP Post-IP Untreated Taxol 12 h Untreated HM LM S-100 HM LM S-100 Pre-IP Post-IP Paclitaxel 100 nM BimEL BimL - + BimEL BimS BimEL BimL BimL BimS BimS CoxIV Ratio 1 1.39 Ratio 1 0.43 BiP/Grp78 Actin Actin MCF-7 MCF-7 MCF-7 Figure S2b

  9. Paclitaxel 100 nM Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ MCF-7 MCF-7 Bcl-2+ - + - + - + - + IP: FLAG IB: Bak IP: FLAG IB: Bcl-2 Bak FLAG-Bcl-2 IP: FLAG IB: Bax FLAG-Bcl-2 Input IB: Bcl-2 Bax Bcl-2 IP: FLAG IB: Puma Puma BimEL IP: FLAG IB: Bim BimL IP: FLAG IB: Bad BimS Bad * BimEL BimL Input IB: Bim BimS IP: FLAG IB: Bmf Bmf Input IB: Bmf Bmf Actin Figure S2c

  10. Paclitaxel 100 nM - + IP: Bcl-2 IB: Bax Bax IP: Bcl-2 IB: Bak Bak Paclitaxel 100 nM - + IP: Bcl-xL IB: Bax MCF-7 Bax IP: Bcl-xL IB: Bak Bak Paclitaxel 100 nM - + IP: Mcl-1 IB: Bax Bax IP: Mcl-1 IB: Bak Bak Figure S2d

  11. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c T47D CoxIV Figure S3a

  12. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c MDA-MB-468 CoxIV Figure S3b

  13. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c BT20 CoxIV Figure S3c

  14. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) IP: Bax 6a7 IB: Bax N20 Bax Bak IP: Bak Ab-2 IB: Bak G23 Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin T47D Figure S4a

  15. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) IP: Bax 6a7 IB: Bax N20 Bax IP: Bak Ab-2 IB: Bak G23 Bak Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin MDA-MB-468 Figure S4b

  16. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Bax IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 Bak Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin BT20 Figure S4c

  17. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin MDA-MB-468 Figure S5a

  18. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin BT20 Figure S5b

  19. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin MCF-7 Figure S5c

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