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Effects of Paclitaxel on Caspase Activation and Apoptosis Pathways in Breast Cancer Cells

This study examines the impact of Paclitaxel on Caspase activation and apoptosis pathways in breast cancer cell lines MCF-7, T47D, MDA-MB-468, and BT20. The research investigates Cleaved Caspase-9, Caspase-8, and Caspase-3 levels at various time points following Paclitaxel treatment. Furthermore, the study delves into the interactions between pro- and anti-apoptotic proteins Bad, Bim, Bcl-2, Bcl-xL, Mcl-1, Bak, Bax, Puma, and Bmf in response to Paclitaxel. The results shed light on the molecular mechanisms underlying Paclitaxel-induced cell death in breast cancer. Figures S1a to S5c present the experimental data and findings.

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Effects of Paclitaxel on Caspase Activation and Apoptosis Pathways in Breast Cancer Cells

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  1. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 MCF-7 Figure S1a

  2. ** * MCF-7 Figure S1b

  3. Paclitaxel 100 nM - + - + - + Bad siRNA - - + + - - Scrambled siRNA - - - - + + Bad Actin BimEL BimL IP: Bcl-2 IB: Bim BimS IP: Bcl-2 IB: Bcl-2 Bcl-2 Input IB: Bcl-2 Bcl-2 Actin MCF-7 Figure S1c

  4. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 T47D Cleaved Caspase-3 Figure S1d

  5. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 MDA-MB-468 Cleaved Caspase-3 Figure S1e

  6. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Cleaved Caspase-9 Caspase-8 BT20 Cleaved Caspase-3 Figure S1f

  7. Paclitaxel 100 nM 0 4 8 12 [h] Bim EL IP: Bcl-xL IB: Bim Bim L Bim S IP: Bcl-xL IB: Bcl-xL Bcl-xL MCF-7 Paclitaxel 100 nM 0 4 8 12 [h] Bim EL IP: Mcl-1 IB: Bim Bim L Bim S IP: Mcl-1 IB: Mcl-1 Mcl-1 Figure S2a

  8. Pre-IP Post-IP Untreated Taxol 12 h Untreated HM LM S-100 HM LM S-100 Pre-IP Post-IP Paclitaxel 100 nM BimEL BimL - + BimEL BimS BimEL BimL BimL BimS BimS CoxIV Ratio 1 1.39 Ratio 1 0.43 BiP/Grp78 Actin Actin MCF-7 MCF-7 MCF-7 Figure S2b

  9. Paclitaxel 100 nM Paclitaxel 100 nM MCF-7 MCF-7 Bcl-2+ MCF-7 MCF-7 Bcl-2+ - + - + - + - + IP: FLAG IB: Bak IP: FLAG IB: Bcl-2 Bak FLAG-Bcl-2 IP: FLAG IB: Bax FLAG-Bcl-2 Input IB: Bcl-2 Bax Bcl-2 IP: FLAG IB: Puma Puma BimEL IP: FLAG IB: Bim BimL IP: FLAG IB: Bad BimS Bad * BimEL BimL Input IB: Bim BimS IP: FLAG IB: Bmf Bmf Input IB: Bmf Bmf Actin Figure S2c

  10. Paclitaxel 100 nM - + IP: Bcl-2 IB: Bax Bax IP: Bcl-2 IB: Bak Bak Paclitaxel 100 nM - + IP: Bcl-xL IB: Bax MCF-7 Bax IP: Bcl-xL IB: Bak Bak Paclitaxel 100 nM - + IP: Mcl-1 IB: Bax Bax IP: Mcl-1 IB: Bak Bak Figure S2d

  11. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c T47D CoxIV Figure S3a

  12. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c MDA-MB-468 CoxIV Figure S3b

  13. Cyto Mito 0 4 8 12 24 48 0 4 8 12 24 48 (hr) Cytochrome c BT20 CoxIV Figure S3c

  14. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) IP: Bax 6a7 IB: Bax N20 Bax Bak IP: Bak Ab-2 IB: Bak G23 Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin T47D Figure S4a

  15. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) IP: Bax 6a7 IB: Bax N20 Bax IP: Bak Ab-2 IB: Bak G23 Bak Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin MDA-MB-468 Figure S4b

  16. 100 nM Paclitaxel 0 4 8 12 24 48 (hr) Bax IP: Bax 6a7 IB: Bax N20 IP: Bak Ab-2 IB: Bak G23 Bak Input IB: Bax N20 Bax Input IB: Bak G23 Bak Actin BT20 Figure S4c

  17. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin MDA-MB-468 Figure S5a

  18. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin BT20 Figure S5b

  19. Paclitaxel 100 nM - + - + - + Puma siRNA - - + + - - Scrambled siRNA - - - - + + Puma Actin MCF-7 Figure S5c

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