Assessment of Root Tip Epidermal Viability Following Inoculation with T3Es

May 17, 2025

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The viability of root tip epidermis cells was evaluated after inoculation with different T3Es mutants. Mutants like GMI1758 and GRS138 exhibited specific effects, indicating their potential roles in altering epidermal integrity. Findings suggest variations in T3Es impact on phenotype, highlighting complex mechanisms influencing epidermal responses.

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Assessment of Root Tip Epidermal Viability Following Inoculation with T3Es

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Supplemental data. Figure 1. A17 root tip epidermal viability scored after inoculation with GMI1000 and all tested T3Es mutants at 3 dpi. Score 0 was attributed to intact epidermis seen T3SS defective mutant controls (GMI1402) and score 4 corresponded to a fully altered epidermis observed with GMI1000. avrA(GMI1758) and gala7 (GRS138) mutants showed a median score of 3 and 2,5 respectively. Root tip epidermis cell death seemed to imply other T3Es having minor effects on the phenotype. Three biological repeats included 10 individual plants per repeat.