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Understanding Retrotransposition in L1 Cycle

This research delves into the intricate process of L1 retrotransposition - from mRNA export and translation to cDNA insertion into the genome via target-primed reverse transcription (TPRT). The study also explores why some insertions fail, yielding non-functional L1 copies. Follow the journey of L1 RNA in the cell's nucleus and cytoplasm as it undergoes reverse transcription and integration into the genome. Source: PEI and schaft.net 8 Relevant

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Understanding Retrotransposition in L1 Cycle

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  1. Figure: L1 retrotransposition cycle L1 mRNA (red) is exported into the cytoplasm, translated, and L1-encoded proteins (L1 ORF1p, L1 ORF2p) bind to their own mRNA (cis preference) and form ribonucleoprotein (RNP) complexes which are reimported into the nucleus. Subsequently, L1 RNA is reverse transcribed and the cDNA is inserted into the genome by a mechanism termed target-primed reverse transcription (TPRT). Frequently, reverse transcription fails to proceed to the 5’ end, resulting in truncated non-functional L1 de novo insertions. Source: PEI http://www.pei.de/nn_156666/EN/research/research-groups/praesident-research-en/pr-retroelements-en/pr-retro1-en/pr-retro1-node-en.html?__nnn=true

  2. http://www.schaft.net/n00bs/2008/05/13001554.html

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