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Chem/Biol 473 S. Schultze and G. Prody Figure 5-21 The central dogma of molecular biology. Page 93 How do RNA and DNA differ? Figure 5-6 Bacteriophages attached to the surface of a bacterium. Page 84 Figure 5-7 Diagram of T2 bacteriophage injecting its DNA into an E. coli cell.

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Chem/Biol 473

S. Schultze and G. Prody

slide7

You need to be able to draw and name

  • their respective nucleosides and
  • the nitrogen containing bases (what are they)
  • their respective nucleotide mono-, di- and triphosphates

You also need to remember amino acid structures, HH,

MM kinetics, thermodynamics, etc. from 471,2.

slide13

Figure 29-17 Electron micrographs of circular duplex DNAs. Their conformations vary from no supercoiling (left) to tightly supercoiled (right).

Page 1122

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Figure 5-14 Schematic representation of the strand separation in duplex DNA resulting from its heat denaturation.

Page 90

figure 5 15 uv absorbance spectra of native and heat denatured e coli dna
Figure 5-15 UV absorbance spectra of native and heat-denatured E. coli DNA.

Page 90

figure 5 17 variation of the melting temperatures t m of various dnas with their g c content
Figure 5-17 Variation of the melting temperatures, Tm, of various DNAs with their G + C content.

Page 91

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Figure 5-10 X-ray diffraction photograph of a vertically oriented Na+ DNA fiber in the B conformation taken by Rosalind Franklin.

Page 87

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Figure 29-1a Structure of B-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis.

Page 1108

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Figure 29-1b Structure of B-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis.

Page 1109

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Figure 29-2a Structure of A-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis.

Page 1110

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Figure 29-2b Structure of A-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis.

Page 1111

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Figure 29-3a Structure of Z-DNA. (a) Ball and stick drawing and corresponding space-filling model viewed perpendicular to the helix axis.

Page 1112

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Figure 29-3b Structure of Z-DNA. (b) Ball and stick drawing and corresponding space-filling model viewed down the helix axis.

Page 1113

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DNA tutorial link

http://molvis.sdsc.edu/dna/index.htm

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Figure 29-7 The conformation of a nucleotide unit is determined by the seven indicated torsion angles.

Page 1116

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Figure 29-8 The sterically allowed orientations of purine and pyrimidine bases with respect to their attached ribose units.
figure 29 10b nucleotide sugar conformations b the c2 endo conformation which occurs in b dna
Figure 29-10b Nucleotide sugar conformations. (b) The C2-endo conformation, which occurs in B-DNA.

Page 1117

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Figure 5-13

Demonstration of the

semiconservative

nature of DNA

replication in E. coli

by density gradient

ultracentrifugation.

Page 89