1. Protein Structure

2. Biology/Chemistry of Protein Structure Primary Secondary Tertiary Quaternary Assembly Folding Packing Interaction S T R U C T U R E P R O C E S S

3. Primary Structure - Sequence

4. FOLDING

5. Basic Secondary structure α-helix β-sheet Secondary structures, α-helix and β-sheet, have regular hydrogen-bonding patterns.

6. 1’ > 2’ > 3’ > 4’ > … Tertiary structure Quaternary structure

7. X - Ray X-ray Diffraction Pattern for a Protein

8. X-Ray

9. NMR – there is no right answer

10. THE database of experimentally-derived structures of proteins, nucleic acids, and complexes • Access to structural information • Visualization • Analysis

11. PDB Growth and Content Macromolecules: Proteins Nucleic acids Carbohydrates Protein-nucleic acid complexes Protein-carbohydrate complexes Experimental Methods: X-Ray Crystallography NMR more…

12. PDB Homepage

13. An Example – 2ARA

14. Search Methods

15. Advanced Search

16. Sequence Search

17. Example Results