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Advanced Biotechnology Applications: Cloning, Stem Cells, and DNA Manipulation

Explore cutting-edge biotechnological techniques such as mammalian cloning, somatic cell nuclear transfer, stem cells, gene cloning, and DNA manipulation through EcoRI restriction enzymes, electrophoresis, and DNA fingerprinting. Learn about variations in DNA, gene splicing, and bacterial defense mechanisms against viral DNA.

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Advanced Biotechnology Applications: Cloning, Stem Cells, and DNA Manipulation

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  1. Biotechnology

  2. Mammalian Cloning Somatic cell nuclear transfer

  3. Stem Cells

  4. AATTCGGCCATATACG GCCGGTATATGCTTAA Desired Gene Restriction Sites for EcoRI Restriction Enzymes • Bacterial defense against viral DNA • Cut DNA at specific sequences CCTTTG AATTCCCAGAATC GGAAACTTAA GGGTCTTAG

  5. Electrophoresis • Separation of molecules based on size • Negatively charged DNA molecules are pulled through a gel by an electrical field • Smaller molecules travel faster and farther

  6. DNA Fingerprinting Variations in DNA Variations in restriction sites Variations in fragment sizes Variations in electrophoresis bands

  7. Poop Walter Jordan

  8. GeneCloning Gene Splicing Transformation Binary Fission

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