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Comprehensive Guide: Inhibition of Murein Biosynthesis

Explore the detailed tutorial by Willie Claiborne Brown, Ph.D., covering the complex process of murein biosynthesis, including steps, reactions, and effects of inhibitors like D-Cycloserine and Beta-Lactam antibiotics.

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Comprehensive Guide: Inhibition of Murein Biosynthesis

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  1. Inhibition of Murein Biosynthesis Tutorial by Willie Claiborne Brown, Ph.D. Professor Emeritus University of California, San Diego January, 2001 WCB

  2. Formation of Amino Sugars NAG UDP NAM UDP Fructose-6-P Glutamine(NH2) Acetyl CoA (NAG) N-Acetylglucosamine UTP P Phosphoenol Pyruvate(Lactyl) Reduction Steps Other Cell Reactions Murein Biosynthesis

  3. Sequence of Events in Murein Biosynthesis NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG P UDP NAM P NAM P-P Bactoprenol Bactoprenol Bactoprenol Section of Growing Murein NAM NAM Bactoprenol P NAM NAM P-P Bactoprenol P-P NAM UDP NAM 6 5 ATP Cytoplasm [Alanine Branch] 7 ATP ATP 2-4 ATP ATP 1 UDP P UMP 9 8 13 Membrane 10 11 12 14 Exterior [Crosslinking Step]

  4. Inhibition of NAM Formation NAG UDP Fructose-6-P Glutamine(NH2) Acetyl CoA N-Acetylglucosamine (NAG) UTP P Phosphoenol Pyruvate(Lactyl) Reduction Steps Other Cell Reactions Fosfomycin Murein Biosynthesis

  5. WCB Effect of D-Cycloserine on Murein Biosynthesis NAG NAG NAG NAG NAG NAG Section of Growing Murein NAM NAM NAM UDP NAM 5 Cytoplasm [Alanine Branch] 7 ATP 2-4 ATP ATP 1 9 8 13 Membrane 10 11 12 14 Exterior [Crosslinking Step]

  6. Effect of Bacitracin on Murein Biosynthesis NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG P UDP NAM P NAM P-P Bactoprenol Bactoprenol Bactoprenol Section of Growing Murein NAM NAM Bactoprenol P NAM NAM P-P Bactoprenol P-P NAM UDP NAM 6 5 ATP Cytoplasm [Alanine Branch] 7 ATP ATP 2-4 ATP ATP 1 UDP UMP 9 8 13 Membrane 10 11 12 14 Exterior WCB [Crosslinking Step]

  7. Effect of Beta Lactam Antibiotics onMurein Biosynthesis NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG NAG P UDP NAM P NAM P-P Bactoprenol Bactoprenol Bactoprenol Section of Growing Murein NAM NAM Bactoprenol P NAM NAM P-P Bactoprenol P-P NAM UDP NAM 6 5 ATP Cytoplasm [Alanine Branch] 7 ATP ATP 2-4 ATP ATP 1 UDP P UMP 9 8 13 Membrane 10 11 12 14 WCB Exterior [Crosslinking Step]

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