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Ribosomes and Protein Synthesis

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Ribosomes and Protein Synthesis

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  1. Ribosomes and Protein Synthesis

  2. Learning Objectives • Identifythe genetic code and explain how it is read. • Summarizethe process of translation. • Describethe central dogma of molecular biology.

  3. The Genetic Code RNA has four bases: adenine, cytosine, guanine, and uracil. These bases form a “language”: A, C, G, and U.

  4. The Genetic Code: Codons The genetic code is read in three-letter groupings called codons. A codon is a group of three nucleotide bases in messenger RNA that specifies a particular amino acid. AAC UCU AUG

  5. Genetic Code Table There are 64 possible three-base codons in the genetic code.

  6. Reading Codons Start at the middle of the circle with the first letter of the codon and move outward. CAC = Histidine

  7. Start and Stop Codons The methionine codon AUG serves as the “start” codon for protein synthesis. There are three “stop” codons. UAA, UAG, and UGAare “stop” codons AUG = methionine = “start” codon

  8. Translation Transcribed mRNA directs the translation process. Translation is the process that makes proteins using the copy of the DNA code on the mRNA.

  9. Translation: Transfer RNA To start translation, tRNA molecules bind to mRNA codons, carrying amino acids with them. anticodon

  10. Translation: The Polypeptide Assembly The ribosome helps form a peptide bond. It breaks the bond holding the first tRNA molecule to its amino acid.

  11. Translation: Completing the Polypeptide The ribosome reaches a stop codon, releasing the newly synthesized polypeptide and the mRNA molecule, completing the process of translation.

  12. Roles of RNA in Translation All three major forms of RNA—mRNA, tRNA, and rRNA—are involved in the process of translation.

  13. The Molecular Basis of Heredity The central dogma of molecular biology is that information is transferred from DNA to RNA to protein.

  14. Gene Expression When a gene (segment) of DNA code is used to build a protein, scientists say that gene has been expressed.