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A. V. Virus. 1 h  1 h 18 - 19 h 45 min each step. Fluorescence staining. I. Inhibitor. Anti-nucleoprotein monoclonal Ab conjugated mouse IgG. + MgCl 2. Treatment. Viral adsorption. Ex336/Em490. Pretreatment. β-galactosidase labeled anti-mouse IgG. 4-Methylumbelliferyl galactoside.

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A

V

Virus

1h  1h18 - 19h45mineach step

Fluorescencestaining

I

Inhibitor

Anti-nucleoprotein monoclonal Ab conjugated mouse IgG

+ MgCl2

Treatment

Viral adsorption

Ex336/Em490

Pretreatment

β-galactosidase labeled anti-mouse IgG

4-Methylumbelliferyl galactoside

V+I

4-Methylumbelliferyl products

MDCK

Oseltamivir

carboxylate, mM

B

PVP-I, mg/ml

MUーGAL

MUーGAL

Relative inhibition, %

Relative virus yield, %

MU

MU

A/Bel/42

A/WS/33

A/Aichi/2/68

A/PR/8/34

A/WSN/33

A/DK/HK/313/78

A/DK/HK/36/4

A/Memphis/1/71

A/DK/HK/677/1

A/DK/HK/313/78

A/USSR/92/77

A/DK/HK/23/76

A/Memphis/1/71

A/DK/HK/92/76

A/Texas/36/91

HumanAvianHumanAvianAvian Human Avian

H1N1H3N2H5N3H9N2H3N2H5N3