Fluorescence staining - PowerPoint PPT Presentation

The Complete Guide to Antibody Detection by Immunofluorescence Technique

Immunofluorescence technique can be used in the detection of hybridoma antibodies of various antigens, such as cellular antigens (including bacterial and animal cells), viral antigens in infected cells, and membrane antigens.

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Relative virus yield, %

A. V. Virus. 1 ｈ　１ h 18 - 19 h 45 min each step. Fluorescence staining. I. Inhibitor. Anti-nucleoprotein monoclonal Ab conjugated mouse IgG. + MgCl 2. Treatment. Viral adsorption. Ex336/Em490. Pretreatment. β-galactosidase labeled anti-mouse IgG. 4-Methylumbelliferyl galactoside.

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