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Zebrafish , Danio rerio , Husbandry. Round Table Discussion. Zebrafish Development and Genetics MBL 2012. Zebrafish International Resource Center. Central Repository Distribution of Lines & Reagents Health & Husbandry Services. University of Oregon.

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zebrafish danio rerio husbandry

Zebrafish, Daniorerio, Husbandry

Round Table Discussion

Zebrafish Development and Genetics

MBL 2012

zebrafish international resource center
Zebrafish International Resource Center
  • Central Repository
  • Distribution of Lines & Reagents
  • Health & Husbandry Services

University of Oregon

environmental and water quality parameters
Environmental and Water Quality Parameters
  • Lighting: 14 hours/day
  • Temperature: 28.5˚C (22-30˚C tolerated)
  • pH: 7.2 to 7.6
  • Conductivity: 500 -1500 µS
  • Ammonia: 0 ppm
  • Nitrite: 0 ppm
  • Nitrate: < 10 ppm
  • Exchange rate: 10% per day (RO water)
feeding
Feeding
  • Larval Diets
    • Dry - micro-capsule
    • Live - Paramecia, Artemia naupli, rotifers
  • Adult Diets
    • Dry - Flake, pellet, crumble, freeze dried
    • Live - Artemia (brine shrimp)
    • Varied diet recommended
  • 2-4 feedings/day
    • Do Not Overfeed
larval culture
Larval Culture
  • Nursery care 3+ weeks
  • Feeding based on larval size
  • Water changes
    • Hand siphoning
    • Continuous flow
  • Lab specific protocols
    • Day 0 to 4: 25-50 embryos per dish
    • Day 5: transferred to tanks and fed

(paramecia, rotifers, powdered food, artemia, or other)

      • Day 5-9: <100 to 250 micron food
      • Day 10-21: 100 to 500 micron food
      • Day 21-25: transferred to aquariums, 150 to 500 micron food
breeding
Breeding
  • Natural Crosses
    • Pairs or small groups
    • Embryo collection
      • Special breeding tanks
      • Marbles
  • In Vitro Fertilization
    • Synchronized development of embryos
  • Do Not Over Breed
    • Allow 1-2 weeks between breeding events
biosecurity quarantine
BiosecurityQuarantine
  • All fish from outside sources
  • Isolated from central facility
    • Tanks, racks, water system
    • Equipment, nets, glassware, etc.
  • Observation of adult fish
    • Observe adults 2-3 weeks prior to breeding
  • Euthanatize or treat sick fish
  • Surface sanitize embryos
    • Highly recommended
internal biosecurity
Internal Biosecurity
  • General Practices
    • Remove sick and dead fish ASAP
    • Minimize transfer of fish and water

between tanks

    • Avoid disease spread from breeding/

out-crossing to wild-types

    • Use nets in one tank only
    • Sanitize equipment between use
  • Sentinel Fish
    • Exposure to return (dirty) water
    • Observed daily
    • Histopathology every 3-6 mo.
contact information
Contact Information

Zebrafish International Resource Center

University of Oregon

Web Address: www.zebrafish.org

April Freeman, Facility Manager: april@zebrafish.org

General Email: zirc@zebrafish.org

ZIRC is supported by grant P40 RR12546 from the NIH-NCRR.

Tufts University

Boston

Caitlin Stewart-Swift, Zebrafish Facility Supervisor: Caitlin.Stewart_swift@tufts.edu