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In Vitro Limiting Dilution Sphere-Forming Assay: Temozolomide-Resistant LN229-TMZR

LN229-TMZR generates one tumor sphere in 22.1 cells while LN229 does so in 79.9 cells in the assay, indicating resistance to temozolomide. The data from the 96-well plate seeding density experiments are presented, revealing significant differences between the two cell lines.

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In Vitro Limiting Dilution Sphere-Forming Assay: Temozolomide-Resistant LN229-TMZR

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  1. YeomSupplementary Fig.8    P= 0.0005 P= 0.04 Supplementary Figure 8. In vitro limiting dilution sphere-forming assay. Temozolomide-resistant LN229-TMZR can generate one tumor sphere in 22.1 cells whereas LN229 can generate one sphere in 79.9 cells (http://bioinf.wehi.edu.au/software/elda). Cells were plated into 96-well plates with various seeding densities (5-500 cells per well, 14 wells per each condition) and were incubated at 37C for 7 days. Data shown are the mean number  SD of spheres per indicated number of inoculated cells. *, P < 0.0001 (vs LN229).

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