Tuesday 9/20/11 What do you think is the most common way that microscopes and or slides are damaged or broken?
Damage to scopes and slides usually occurs during focusing or cleaning the lens. Focusing incorrectly can break slides & lenses. Improper cleaning can reduce resolution of lenses
Resolution The resolution of an optical microscope is defined as the shortest distance between two points on a specimen that can still be distinguished by the observer or camera system as separate entities. An example of this important concept is presented in the figure below (Figure 1), where point sources of light from a specimen appear as Airy diffraction patterns at the microscope intermediate image plane. Resolution refers to the smallest distance between 2 points that can still be distinguished by the observer. CAUTION – ONLY USE LENSE PAPER to clean the lenses. Other products will scratch the coating and decrease the resolving power of the lens.
Demonstration • letter “ bare eye” • When finished drawing place on • Microscope so the “e” is right • side up. • how to focus • how to find the power of magnification • ocular lens x objective lens = power
Letter “e” 4x10 = 40 power Letter “e” bare eye Letter “e” 10x__=__ n Letter “e’ ___x___ =___ _______ __x__=__ Microscope Observations Threads ______ __x__=__ ______ __x__=__ Cork High Power __x__=__
Microscope Observation Rubric Heading___________________3______ 8 drawings________________24______ Color____________________ 3_______ Level of magnification filled in__6______ Total_____________________36______
You will work in teams of 2 • 1 person gets the microscope • The other gets colored pencils and the • first side = “e” • When finished drawing place on • Microscope so the “e” is right • side up.
Microscope Lab Directions • Letter “e” draw it without using microscope • draw “e” using low power • draw “e” using high power • Return the “e” slide and get a “cork” slide • a. draw “cork” using high power • Return the “cork” slide and get a “thread” slide • a. draw “threads showing what color is on top... • Return the “thread” slide and prepare a wet • mount slide using directions on back of “Light Microscope” handout. Use the sample of tank sludge for the water and sample. • a. draw at low and high power (find a monster)