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Apoptosis Induction by SIRT1 siRNA and AROS siRNA in Cancer Cells

Investigating the fold apoptosis induction in cancer cells with SIRT1 siRNA and AROS siRNA treatment. Analysis of mRNA expression in HCT116, MCF7, WI-38, and MCF10A cell lines. Etoposide, TSA, and UV-C treatment effects on p53 acetylation examined.

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Apoptosis Induction by SIRT1 siRNA and AROS siRNA in Cancer Cells

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  1. Supplemental Table 1: Knight et al – Supplementary Table

  2. A B n.s. n.s. ** Fold apoptosis induction - - + + SIRT1 siRNA AROS 2 siRNA Mock - - AROS 1 siRNA + + Fold apoptosis induction Knight et al – Supplementary Figure 1

  3. HCT116 mRNA expression (%) SIRT1 mRNA Lamin AC mRNA AROS mRNA siRNA HCT116 p53-/- MCF7 mRNA expression (%) mRNA expression (%) SIRT1 mRNA SIRT1 mRNA Lamin AC mRNA Lamin AC mRNA AROS mRNA AROS mRNA siRNA siRNA WI-38 MCF10A mRNA expression (%) mRNA expression (%) SIRT1 mRNA SIRT1 mRNA Lamin AC mRNA Lamin AC mRNA AROS mRNA AROS mRNA siRNA siRNA Knight et al – Supplementary Figure 2

  4. A Etoposide and TSA Control Control UV-C Treatment p53 50kDa K382Ac 50kDa * β-actin 37kDa B *** *** *** *** Fold apoptosis vs. Mock Mock Mock SIRT1 Mock Mock SIRT1 AROS AROS siRNA UV-C irradiation Etoposide and TSA Condition Knight et al – Supplementary Figure 3

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