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T T T T A G T G T G. XTC.UC1. T T T T A G T T G T G. Control. PTEN. A. B. XTC.UC1. Control. PTEN. b- ACT.

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## T T T T A G T G T G

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**T T T T A G T G T G**XTC.UC1 T T T T A G T T G T G Control PTEN A B XTC.UC1 Control PTEN b-ACT PTEN-null status of XTC.UC1 cells. (A) Electropherogram showing the hemizygous c.210delT within exon 4 of the PTEN gene in XTC.UC1 cells compared to a wild-type control (lower panel). The wild-type c.210delT is underlined. (B) Western blot for PTEN confirming lack of the full-length protein in XTC.UC1 cells compared to a control. Beta-actin was used as a loading control.

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