Cell Characterization & Measurement of Viability - PowerPoint PPT Presentation

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Cell Characterization & Measurement of Viability

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  1. Cell Characterization & Measurement of Viability Dr Alok Tripathi aquaimmuno@yahoo.co.in +919915546129 Department of Biotechnology

  2. 6x106 Note the lag phase before the cells start to grow, the log phase of growth, and the beginning of the plateau phase when contact-inhibited cells will cease growing. The population doubling time should be calculated during log phase growth and is 20 hr for this cell line (RL-65) 1x106 1x105 1x104

  3. Using the Hemacytometer • Materials • 1. Trypsinized cell cultures • 2. Improved Neubauerhemacytometer with coverslip • 3. Tally counter • 4. 0.4% trypan blue in PBS • 5. Pasteur pipettes • 6. Microscope