Hose: Day 1, ~1 PM - PowerPoint PPT Presentation

Presentation Transcript

Depicted on the following slides are water samples taken from scuba diving rinse tanks at a dive facility on Bonaire, Netherlands Antilles October, 2007, for 3 days.

Equipment rinse tank “A” was drained, cleaned with bleach and rinsed each morning, before filling with water and sampling (~8:00 AM)
Equipment rinse tank “B” was drained and rinsed each morning, before filling with water and sampling (~8:00 AM)
Water samples were taken from both equipment rinse tanks at ~1:00 PM and ~5:00 PM
Water samples were taken from a regulator rinse tank daily at ~8:00 AM, ~1:00 PM and ~5:00 PM, but we did not know when this tank was drained and refilled (by dive facility personnel and other divers)
Water samples were taken daily from the hose used to fill all rinse tanks
On two days water samples were taken from buckets on dive boats used to rinse/store masks
0.25 ml of each water sample was spread on Terrific broth-agar plates; plates were incubated at 37oC overnight and photographed

On all days, water samples from the hose (throughout the day) and from equipment rinse tanks “A” and “B” at ~8:00 AM, after draining, cleaning with bleach (“A”) or not cleaning with bleach (“B”), rinsing and refilling did not produce any bacterial colonies. Representative plates are shown below.

Hose: Day 1, ~1 PM

Hose: Day 2, ~8 AM

Hose: Day 3, ~8 AM

Tank “B”: Day 1, ~8 AM

Tank “A”: Day 3, ~8 AM