Construction and Analysis of Plasmid pRB71 for lacZ and actP Gene Fusion

DESCRIPTION

This document outlines the creation of plasmid pRB71, which consists of a fusion between the lacZ gene and the actP (acetate permease) gene. The fusion sequence includes a 450 bp upstream region preceding the first open reading frame (ORF) of the operon, incorporating the complete acs (acetyl-CoA synthetase) gene along with the first 317 bp of the actP gene. Further details regarding plasmid construction and the implications of this genetic arrangement are provided, aiding in the understanding of gene expression regulation in bacteria.

Download

1 / 1

May 15, 2026

Download Presentation

Construction and Analysis of Plasmid pRB71 for lacZ and actP Gene Fusion

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

1 kb SUPPLEMENTARY MATERIAL S 1. Fusion of lacZ to actP (acetate permease) to give plasmid pRB71. The fusion sequence comprises 450 bp upstream of the first ORF of the operon, the entire acs (acetyl-CoA synthetase) and the first 317 bp of actP (See plasmids construction for further details). hypothetical protein hypothetical protein acs actP pRB71 P lacZ P