1 / 1

Comparative Plate Growth Assay of M. grisea Strains on Different Media Conditions

Explore the impact of various carbon sources on the growth and cutin-degrading activities of M. grisea strains using a plate growth assay. Results indicate differences in extracellular cutin-degrading activities among Guy11, cut2, and cut2/CUT2 strains.

dafydd
Download Presentation

Comparative Plate Growth Assay of M. grisea Strains on Different Media Conditions

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Guy11 cut2cut2/CUT2 A B C D E F Supplementary figure 2. Plate growth assay of M. grisea strains Guy11, cut2 and cut2/CUT2 on MM incubated at 24oC for 10 days with a 12 h light-dark cycle. (A) MM with1% glucose;(B) MM without glucose; (C) MM with 0.2% cutin; (D)MM with 500 mg L-1PCL; (E) MM with 0.1% Tween 80; (F) Extracellular cutin-degrading activities of Guy11, cut2 and cut2/CUT2 strains visualized by yellow haloes formed on addition of PNB substrate to 10 day old MM plates, carrying 0.2% plant cutin as sole carbon source. The yellow halo formed by cut2 is smaller and fainter compared with the halo seen in Guy11 and cut2/CUT2 strains, indicating reduced extracellular cutin-degrading activity of the cut2 mutant.

More Related