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Overview of Instruments/Services at the Analytical Instrumentation Core

Overview of Instruments/Services at the Analytical Instrumentation Core. Matthew Au Array Service Manager December 1 st , 2015. Overview of AIC Instruments. Services Offered. Bio-Rad Supply Center qRT -PCR Array Service Lipid Analysis Service Fatty Acids/Monosaccharides

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Overview of Instruments/Services at the Analytical Instrumentation Core

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  1. Overview of Instruments/Servicesat the Analytical Instrumentation Core Matthew Au Array Service Manager December 1st, 2015

  2. Overview of AIC Instruments

  3. Services Offered • Bio-Rad Supply Center • qRT-PCR Array Service • Lipid Analysis Service • Fatty Acids/Monosaccharides • Protein/Cytokine Array Service • Luminex Platform • Multiplexing

  4. Protein/Cytokine Arrays as an Extension of qRT-PCR • Logical extension of PCR Arrays that can verify that changes at the mRNA level (gene expression) correspond to changes at the protein level • Can also be used to identify the presence and amount of various proteins/cytokines in a sample • Viewed as an alternative to running ELISAs

  5. Brief Overview of the Workflow • Researchers will submit their samples • Plasma, serum, cell supernatants, etc. • Purchase kits from various vendors • EMD Millipore, R&D Systems, Bio-Rad, etc. • Kits contain magnetic beads that already have capture antibodies and are fluorescently coated Luminex Magpix Instrument

  6. Miniaturization of Protein Arrays • Incorporated Curiox Biosystems’ DropArray Technology • Typically run the assays on a regular 96-well microtiter plate using handheld magnet (bottom left) • Conversion has allowed a 5-fold reduction in the amount sample volume and reagents required Conventional MTP and Handheld Magnet Wall-Less Plate and Automated Plate Washer

  7. Advantages of Miniaturization • Reduces total assay costs 60-80%

  8. Sample Data Mouse 5-Plex Kit from EMD Millipore

  9. Different Kits Available • Cancer • Cardiovascular • Cell Signaling • Cellular Metabolism • Immunology • Metabolism & Endocrinology • Neuroscience • Stem Cells • Toxicity • Custom

  10. Contact Us! • Array Service Manager Matthew Au mau115@bu.edu 617-414-2964 Central Lab • Director of AIC Lynn Lingyi Deng, Ph.D., lynndeng@bu.edu 617-638-6920 office

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