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DNA Amplification for Targeted Loci Analysis

Learn how PCR is used to make numerous copies of specific loci of interest in DNA samples. Explore the process of amplification, from selecting target loci to tagging them with fluorescent dyes for analysis.

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DNA Amplification for Targeted Loci Analysis

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  1. How they make a scrimillion copies of the DNA

  2. D3S1358 D18S51 D5S818 D7S820 D8S1179 vWA D21S11 Amelogenin FGA D13S317 Find the areas of interest

  3. In the old days, they cut before and after

  4. D3S1358 D18S51 D5S818 D7S820 D8S1179 vWA D21S11 Amelogenin FGA D13S317

  5. PCR me, ASAP! So we’ve removed the DNA from the cells, now what?

  6. P C R • PCR = Polymerase chain reaction. • Used to make scrimillions of copies of the loci of interest.

  7. bases = nucleotides

  8. CGACATGTCAT TACGTAGGCTAACTGCTACAGT GATAGCAGCGTCCGATCTAGCA

  9. C G A T C G A T

  10. C G A T C G A T

  11. C G T A C G A T

  12. C G A T G C G C C G T A T A T A C G C G A T A T

  13. C G A T G C G C C G T A T A T A C G C G A T A T

  14. C G T A G C G C C G T A T A T A C G C G A T A T

  15. This is amplification. Technically, this is not a science term either. Amplify: Root words Amplus, large + facere, to make* So we can just keep splitting it open and adding bases and make millions and millions of copies. *Webster’s New World Compact School and Office Dictionary, at 15, (1989)

  16. The loci we care about are tagged with a flourescent dye. . . Holt Science and Technology, Life Science, p. 137

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