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What did you learn from surfing FlyBase?

What did you learn from surfing FlyBase? Why do the inversions in Balancer chromosomes greatly reduce the frequency of crossing over in meiosis?. Autonomous vs non autonomous. Nonautonomous. Domineering - mutant cells disrupt the development of neighboring wild type cells.

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What did you learn from surfing FlyBase?

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  1. What did you learn from surfing FlyBase? • Why do the inversions in Balancer chromosomes greatly reduce the frequency of crossing over in meiosis?

  2. Autonomous vs non autonomous

  3. Nonautonomous • Domineering - mutant cells disrupt the development of neighboring wild type cells. • Submissive - wild type neighbors rescue mutant cells.

  4. FLP/FRT can also be used to remodel chromosomes.

  5. RNAi in flies ds RNA in cultured cells. http://flyrnai.org/RNAi_goals.html

  6. RNAi in flies • Express a fold back RNA from a transgene. • This results in tissue/temporal specific gene knockdowns. • Collections of transgenic flies are available that contain transgenes to knockdown the expression of most fly genes.

  7. Gene targeting • Until recently not available in flies. • One difficulty is that one cannot select for a targeted cell. • A technique has now been developed that has been shown to work on a number of genes.

  8. Technique requires first getting a germ line transformant for the knock out construct. • The DNA for targeting is excised by the activity of the FLP/FRT site specific recombination system. • The excised circle is then cut by the induced expression of a very rare cutting RE. • High rate of homologous targeting.

  9. In Vivo Imaging • A number of developmental stages/tissues provide opportunities for in vivo imaging.

  10. Genetic Screens • Recessive lethal mutations

  11. Flp/FRT • High efficiency of FLP/FRT clone induction has allowed new types of mutant screens.

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