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Optimizing Whole Blood Sample Preparation for Gene Expression Microarray Experiments

This document discusses the methods for preparing whole blood samples for gene expression microarray experiments, focusing on the use of PAXgene tubes for effective RNA extraction and preservation. Key techniques include biotin labeling, RNase treatment, and post-incubation DNase treatment to enhance RNA quality for analysis. The research explores the impact of longer incubation times on sample quality and underscores the importance of meticulous sample preparation for successful microarray outcomes. This work references studies by Wang et al. on enhancing RNA's stability and integrity prior to analysis.

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Optimizing Whole Blood Sample Preparation for Gene Expression Microarray Experiments

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  1. Whole Blood Sample Prep for gene expression microarray experiments 10/19/06 Research papers and Tech Notes

  2. Bioanlyzer trace for cRNA after biotin labeling PAXgene tubes + RNase H

  3. Wang et al Clin Biochem 2004 Sep; 37(9):741-4 Longer incubation time

  4. PAXgene tubes + off column DNase treatment

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