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Li_Supplementary Figure 6

Li_Supplementary Figure 6. GST-UIM1+2. GST. M r (Kd). 14.5. IB: Anti-Ub. 6.0. 37.1. 10% input. 25.9. Coomassie. Li_Supplementary Figure 7. A DA1-related proteins in Arabidopsis. DA1. DAR1. DAR2. DAR3. DAR4. DAR5. DAR6. DAR7. B Homologs of DA1. B. A. Seed weight (mg).

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Li_Supplementary Figure 6

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  1. Li_Supplementary Figure 6 GST-UIM1+2 GST Mr(Kd) 14.5 IB: Anti-Ub 6.0 37.1 10% input 25.9 Coomassie

  2. Li_Supplementary Figure 7 A DA1-related proteins in Arabidopsis DA1 DAR1 DAR2 DAR3 DAR4 DAR5 DAR6 DAR7 B Homologs of DA1

  3. B A Seed weight (mg) Petal area (mm2) Col-0 da1-1 da1-ko1 Col-0 da1-1 da1-ko3 da1-ko2 da1-ko1 da1-ko2/Col-0 F1 da1-ko1/Col-0 F1 da1-ko3/Col-0 F1 da1-ko3/da1-1 F1 da1-ko1/da1-1 F1 da1-ko2/da1-1 F1 da1-1/Col-0 F1 da1-ko1/Col-0 F2 seeds da1-ko1/da1-1 F2 seeds 35S::DA1R358K#5 da1-1 C Col-0 3.5 DA1 5 3 4.5 rRNA 4 2.5 3.5 2 3 1.5 2.5 2 1 1.5 0.5 1 0.5 0 0 Li_Supplementary Figure 8

  4. C Ler 7 da1-1Ler bb-1 6 bb-1da1-1Ler 5 Petal area (mm2) 4 3 2 1 0 4 3.5 3 2.5 2 1.5 1 0.5 0 Col-0 da1-1 eod1-2da1-1 eod1-2 Ler da1-1Ler bb-1 da1-1Ler bb-1 Li_Supplementary Figure 9 A B Seed weight (mg) da1-1 eod1-1da1-1 da1-1 eod1-1da1-1 D E

  5. ant-5da1-1Ler Ler da1-1Ler ant-5 axr1-12 Col-0 axr1-12da1-1Ler da1-1Ler Petal area (mm2) 4.5 4 3.5 3 2.5 2 1.5 1 0.5 0 Li_Supplementary Figure 10 A B 4.5 4 3.5 Petal area (mm2) 3 2.5 2 1.5 1 0.5 0 C D 6 6 5 5 Seed weight (mg) Seed weight (mg) 4 4 3 3 2 2 1 1 arf2-7 0 0 ap2-7 da1-1 da1-1 Col-0 Col-0 ap2-7da1-1 arf2-7da1-1

  6. 100 80 60 40 20 0 100 80 60 40 20 0 6 Col-0 Col-0 Col-0 da1-1 da1-1 da1-1 5 4 3 2 1 0 Li_Supplementary Figure 11 % germination A 0 1µM 4µM ABA 2µM % Green cotyledon B 5 6 7 8 9days Root length (cm) C 0 5µM 10µM 30µM ABA

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