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Stephanie Bachar Matt Luchette

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Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip Technology Toner, et al. 2007. Stephanie Bachar Matt Luchette. Background: Circulating Tumor Cells. CTCs: tumor cells shed into the blood stream Motivation for detection Study biology of metastasis

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Isolation of Rare Circulating Tumor Cells in Cancer Patients by Microchip TechnologyToner, et al. 2007

Stephanie Bachar

Matt Luchette

background circulating tumor cells
Background: Circulating Tumor Cells
  • CTCs: tumor cells shed into the blood stream
  • Motivation for detection
    • Study biology of metastasis
    • Diagnose, detect, monitor cancer in patients without invasive biopsy

The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites

Y Shiozawa, A M Havens, K J Pienta and R S Taichman

motivation for the ctc chip
Motivation for the “CTC-Chip”
  • Shortcomings prior to “CTC-Chip”
    • Accuracy: 20-60%
    • Purity: 0.1%
    • Non-viable cells

Photoacoustic detection of CTCs. Viator lab at University of Missouri

http://web.missouri.edu/~viatorj/tumor.htm

how to use the ctc chip
How to use the “CTC Chip"
  • Flow sample through CTC Chip
  • EpCAM coated microposts
  • Verify with markers

Pressure Control

Blood on Rocker

CTC Chip

Toner 2007

EpCAM

Non-CTCs

CTCs

Micropost

Cytokeratin

CD45

instrument design in silico
Instrument Design: in silico
  • Maximize CTCs detected
    • Flow Velocity
    • Shear Force
  • Vary micropost characteristics
  • Theoretical capture efficiency of 65%

Toner 2007

laboratory results
Laboratory Results
  • 1 target cell/10^9 blood cells
  • Purity of capture ~60% (competitors = 0.1%)
  •  Efficiency of capture ~65% (competitors = 35%)
  • Captured cells were viable and could be further manipulated

Capture purity per type of cancer

Toner 2007

clinical results
Clinical Results
  • Identified CTCs in 99% cancer pts (competitors =  20-60%)
  • no false positives
  • Change in number of CTCs correlated with clinical path
  • No pre-processing required

Change in CTCs captured per mL (red) with lung cancer patient mirrors change in tumor size (blue)

Toner 2007

concerns and next steps
Concerns and next steps
  • How cells used afterwards?
  • How to mass produce chips?
  • Financially viable?
  • How reliable is EpCAM as a caner cell marker?
  • FDA approval

Paper tree-branch glucose and protein assay for urine

technologyreview.com

recap applications
Recap/Applications
  • New diagnostic and prognostic tool for cancer patients
  • Provide alternative to biopsies for areas with low resources
  • Use to isolate and further study CTCs

MRI of 67-year old patient with pancreatic cancer with the pancreatic tumor indicated

imagingeconomics.com

works cited
Works Cited

--imagingeconomics.com

--technologyreview.com

--http://web.missouri.edu/~viatorj/tumor.htm

--The bone marrow niche: habitat to hematopoietic and mesenchymal stem cells, and unwitting host to molecular parasites Y Shiozawa, A M Havens, K J Pienta and R S Taichman

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