slide1 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
MICROSCOPES & MAGNIFICATION PowerPoint Presentation
Download Presentation
MICROSCOPES & MAGNIFICATION

Loading in 2 Seconds...

play fullscreen
1 / 17

MICROSCOPES & MAGNIFICATION - PowerPoint PPT Presentation


  • 122 Views
  • Uploaded on

MICROSCOPES & MAGNIFICATION. COMPOUND LIGHT MICROSCOPE:. USES LIGHT PASSING THROUGH THE SPECIMEN TO ENLARGE IT 2 LENSES OBJECTS APPEAR BACKWARDS & UPSIDE DOWN!. MICROSCOPE PARTS. FOCUSING:. NOT FOLLOWING FOCUSING PROCEDURES CAN SEVERLY DAMAGE THE MICROSCOPE &/OR SLIDE!

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'MICROSCOPES & MAGNIFICATION' - alexavier-jacob


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
compound light microscope
COMPOUND LIGHT MICROSCOPE:
  • USES LIGHT PASSING THROUGH THE SPECIMEN TO ENLARGE IT
  • 2 LENSES
  • OBJECTS APPEAR BACKWARDS & UPSIDE DOWN!
focusing
FOCUSING:
  • NOT FOLLOWING FOCUSING PROCEDURES CAN SEVERLY DAMAGE THE MICROSCOPE &/OR SLIDE!
  • ALWAYS START WITH THE LOWEST MAGNIFICATION AND WORK UP TO A HIGHER MAGNIFICATION!
focusing procedures
FOCUSING PROCEDURES:
  • PLACE THE SLIDE ON THE STAGE & CLIP IT IN TO SECURE
  • ADJUST THE DIAPHRAGM TO THE PROPER AMOUNT OF LIGHT
  • MAKE SURE YOU ARE ON THE LOWEST OBJECTIVE LENS
  • LOWER THE OBJECTIVE USING THE COARSE ADJUSTMENT KNOB
slide7
BRING THE SPECIMEN INTO FOCUS USING THE FINE ADJUSTMENT KNOB
  • CHANGE TO A HIGHER POWER OBJECTIVE
  • USE ONLY THE FINE ADJUSTMENT KNOB AT THIS POINT!
  • NEVER USE THE COARSE ADJUSTMENT AT HIGH POWER – YOU WILL BREAK THE SLIDE/LENS
  • WHEN DONE – STORE THE MICROSCOPES IN THEIR COVERS UNDER THE SINKS
making a wet mount slide
MAKING A WET MOUNT SLIDE:
  • OBTAIN A CLEAN SLIDE & COVER SLIP
  • PLACE A SPECIMEN IN THE MIDDLE OF THE SLIDE (USE TWEEZERS)
  • WITH A DROPPER, PLACE ONE DROP OF LIQUID OVER THE SPECIMEN – DO NOT TOUCH THE DROPPER TO THE SPECIMEN!
  • HOLD THE COVER SLIP BY THE EDGES AT (WITH TWEEZERS)
  • LOWER IT SLOWLY ON AN ANGLE!
slide9
IF AIR BUBBLES ARE TRAPPED UNDER THE COVER SLIP, GENTLY TAP THE EDGE OF THE COVER SLIP WITH THE BLUNT END OF THE TWEEZERS
  • REMOVE EXCESS WATER WITH A PIECE OF PAPER TOWEL (TWEEZERS AGAIN, FOLKS)
  • PLACE THE SLIDE ON THE STAGE & VIEW!
making a wet mount stain
MAKING A WET MOUNT STAIN:
  • MAKE A WET MOUNT SLIDE
  • USING A DROPPER, ADD A DROP OF STAIN ALONG THE EDGE OF THE COVERSLIP
  • TOUCH A PIECE OF PAPER TOWEL TO THE OTHER SIDE OF THE COVER SLIP TO DRAW THE STAIN THROUGH
magnification
MAGNIFICATION:
  • THE ABILITY TO MAKE IMAGES LARGER
  • RESOLUTION: THE SHARPNESS OR CLEARNESS OF AN OBJECT
  • TO CALCULATE TOTAL MAGNIFICATION: MULTIPLY THE MAGNIFYING POWER OF THE OBJECTIVE LENS x THE MAGNIFYING POWER OF THE OCULAR
      • OCULAR = 10x
      • LOW POWER LENS = 4x
      • TOTAL MAGNIFICATION = 40x
dissecting microscope
DISSECTING MICROSCOPE:
  • USED TO MAGNIFY 3 DIMENSIONAL SOLID OBJECTS (FOR DISSECTING)
  • OBJECTS APPEAR RIGHT SIDE UP AND NORMAL!!!!
  • NO SPECIAL SLIDES OR MOUNTS
  • ONLY 1 OBJECTIVE LENS – VERY LOW POWER
electron microscope
ELECTRON MICROSCOPE:
  • USES A BEAM OF HIGHLY ENERGETIC ELECTRONS TO EXAMINE OBJECTS ON A VERY FINE SCALE
  • CAN PRODUCE MAGNIFICATIONS OF MORE THAN 500,000 x THAT OF LIGHT!
  • ONE DISADVANTAGE IS THAT SAMPLES MUST BE VIEWED IN A VACUUM – SO NO LIVING SAMPLES CAN BE STUDIED!
slide16

TRANSMISSION ELECTRON MICROSCOPE

SCANNING ELECTRON MICROSCOPE

slide17

E. COLI BACTERIA

HEAD OF A SMALL BLACK ANT

POLLEN