1 / 11

Ultraviolet/Visible Spectrometry in Quantitative Analysis

This chapter explores the application of UV/Visible molecular absorption spectrometry in quantitative analysis, covering procedural details, wavelength selection, variables influencing absorbance, and the standard addition method. Learn about determining the relationship between absorbance and concentration.

adailey
Download Presentation

Ultraviolet/Visible Spectrometry in Quantitative Analysis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Application of Ultraviolet/Visible Molecular Absorption Spectrometry Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14 Chapter 14

  2. Quantitative Analysis by Absorption Measurement • Scope • Application to absorbing species • Application to non-absorbing species • Procedural Details • Selection of wavelength • Variables that influence absorbance • Cleaning and handling of cells • Determination of the relationship between absorbance and concentration • The standard addition method • Cs = A1CxVx/((A2-A1)*Vx)

  3. Standard Addition Method 1.000 0.800 m =0.03820 Absorbance 0.600 0.400 b = 0.2412 0.200 Vs = -6.35 0.0 10.0 20.0 -10.0 Volume standard solution mL

  4. Two Component Mixture M + N Absorbance N M l’’ l’ Wavelength

  5. es = ep = 0 et >0 es > 0 ep = et = 0 Absorbance Absorbance Volume of titrant Volume of titrant et > ep > 0 es = 0 es > et > 0 ep= 0 es = 0 ep > et > 0 Absorbance Absorbance Absorbance Volume of titrant Volume of titrant Volume of titrant Typical Photometric Titration Curves ep > 0 es = et = 0 Absorbance Volume of titrant

More Related