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Characteristics of the Enterobacteriaceae. Gram-negative rodsGlucose is fermented with strong acid formation and often gasCytochrome oxidase activity is negativeNitrate is reduced to nitrite . Gram\'s Stain for Bacterial Morphology. Crystal violet binds to cell wall peptidoglycan with Gram\'s iodine as a mordantSafranin or basic fuchsin counterstains bacterial cells decolorized by alcohol-acetone.

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