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Lecture 5: Microscopy

Lecture 5: Microscopy. PHYS 430/603 material Laszlo Takacs UMBC Department of Physics. Useful sites: http://www.microscopyu.com/articles/optics/components.html http://em-outreach.ucsd.edu/web-course/toccontents.html. Light microscopy The principle and a commercial scope.

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Lecture 5: Microscopy

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  1. Lecture 5: Microscopy PHYS 430/603 material Laszlo Takacs UMBC Department of Physics

  2. Useful sites: http://www.microscopyu.com/articles/optics/components.html http://em-outreach.ucsd.edu/web-course/toccontents.html Light microscopyThe principle and a commercial scope

  3. Optical microscope (OM) and transmission electron microscope (TEM)

  4. TEM modes:Composite image, bright field image, dark field image, selected area diffraction First image Transmitted and diffracted beams Diffraction pattern Bright field Bright field, transmitted electrons only Dark field Selected area diffraction pattern

  5. Comparing imaging and diffraction in a TEM.

  6. Electron diffraction from amonocrystal polycrystal glass

  7. How do scanning microscopies work? • Measurement generates a value for every location: • Reflectivity of light (scanner) • Ejected electrons (SEM) • Current between tip and surface (STM) • Force between tip and surface (AFM) • Any quantity of interest that can produce useful contrast Interpret numbers as intensities for display or printer. Digital image processing. Image = table of numbers

  8. How do scanning microscopies work? • Measurement generates a value for every location: • Reflectivity of light (scanner) • Ejected electrons (SEM) • Current between tip and surface (STM) • Force between tip and surface (AFM) • Any quantity of interest Interpret numbers as intensities for display or printer. Digital image processing. Image = table of numbers

  9. The principle of the SEM. There is no image formation in the optical sense. This is classical analog system. Modern SEMs record the measured intensities in a computer memory rather then project them directly on a CRT screen. This way image processing is possible before the final image is created. TV does the same.

  10. Measurable effect caused by high-energy electrons

  11. Typical morphological contrast by secondary electrons.It only looks like an illuminated landscape. The contrast comes from how many secondary electrons are generated and how efficiently they are collected by the detector. The illumination comes from above, the detector is n the side.

  12. Magnifications: very different features are seen on different length scales

  13. Depth of field

  14. X-ray analysis

  15. Images of a Lunar rock:(a) Backscattered electrons (depend on Z)(c) Fe X-rays(d) P X-rays(b) Sketch of phases; m = metal tr = trolite, FeS sc = Fe-Ni phosphide wh = phosphate

  16. The principle of STM/AFM

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