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Protein Synthesis. By: Sophie gollan. In this experiment we modelled the structure of DNA and the processes involved in protein synthesis from the information in the DNA. DNA structure. DNA - a double stranded helix molecule which consists of subunits called nucleotides.

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Protein synthesis

Protein Synthesis

By: Sophie gollan



Dna structure
DNA structure the processes involved in protein synthesis from the information in the DNA

  • DNA - a double stranded helix molecule which consists of subunits called nucleotides.

  • Each nucleotide contains a sugar, a phosphate, and a base.

  • There are four bases:

  • adenosine

  • thymine

  • cytosine

  • guanine

  • Alternate sugar and phosphates form the sides, and the bases are connected to the sugars making “rungs” like a ladder.

  • The chemical structure of the bases allow them each to pair up with only one other base, thus they form complementary pairs.

  • The complementary pairs are:

  • Adenosine and thymine

  • Cytosine and guanine


Protein synthesis1
Protein synthesis the processes involved in protein synthesis from the information in the DNA

  • The information about the number, type and sequence of amino acids, needed to make a protein molecule, is found as a code in DNA.

  • The code- a sequence of bases.

  • One gene sequence codes for one polypeptide (a single chain of many amino acids)

  • A set of 3 bases (a codon) codes for one amino acid of a polypeptide.

  • A protein is one or more polypeptides.


Equipment
Equipment the processes involved in protein synthesis from the information in the DNA

  • 42 toothpicks

  • 18 milk bottles cut in half (36 halves) – sugar

  • 18 raspeberry lollies cut in half- phosphate

  • 25 jelly beans cut in half (5 of each 5 colours)- bases:

  • Adenosine- orange

  • Thymine- purple

  • Cytosine- pink

  • Guanine- green

  • Uracil- blue

  • 4 jelly snake, aproxx. 6cm long, different colours

  • A4 white paper representing a cell

  • Colored paper circle, 6cm diameter- a ribosome

  • Clean sharp knife

  • Cutting board

  • Gloves

  • Scissors

  • Marking pen

  • Heinemann Biology textbook


Transcription
Transcription the processes involved in protein synthesis from the information in the DNA

  • A gene length of DNA unwinds in the nucleus. This is the area containing the information about the protein to be made.


  • R the processes involved in protein synthesis from the information in the DNANA polymerase enzyme moves along the exposed single DNA strand linking complementary RNA nucleotides together to form a mRNA strand.

  • RNA contains the base uracil where thymine is found in DNA. (uracil replaces thymine)

  • The ‘start’ codon and the ‘stop’ codon control the length of the mRNA strand



  • ACTIVATION OF AMINO ACIDS: the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together.

  • In the cytoplasm, an enzyme attaches amino acids to tRNA molecules. Each type of amino acid is attached to its specific tRNA.


  • mRNA passing out of the nuclear pores into the cytoplasm the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together.

  • triplet codons of tRNA with amino acids in the cytoplasm of the cell


Translation
TRANSLATION the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together.

  • The start codon (AUG) end of the mRNA strand binds onto a ribosome. A tRNA carrying the amino acid methionine at one end and anticodon (UAC) at the other, binds to the mRNA start codon within the ribosome.


  • A second the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together.tRNA binds to the next codon. Its amino acid links to the polypeptide bond of the first amino acid.

  • The first tRNA is released from the ribosome. The ribosome moves along the mRNA strand one codon at a time. Two tRNAs at a time are temporarily bound within the ribosome and their amino acids linked together

Ribosome

Amino acid forming polypeptide bond (jelly snakes)

mRNA strand

DNA strand

Triplet codon of tRNA


  • A polypeptide chain forms (jelly snakes) the base sequence that will code for the protein. It removes the non-coding regions, introns, while still in the nucleus by splicing the coding regions, exons, together.

Snakes form the polypeptide chain


Polypeptide chain



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