Mycobacterium avium infection in a horse n07 506
Download
1 / 16

Mycobacterium avium infection in a Horse N07-506 - PowerPoint PPT Presentation


  • 103 Views
  • Uploaded on

Mycobacterium avium infection in a Horse N07-506. Ian Hawkins. N07-506 - History. An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis Improved on banamine and lidocaine until 8/25/07. Abdominocentesis revealed elevated WBC count and TP

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Mycobacterium avium infection in a Horse N07-506' - quemby-raymond


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Mycobacterium avium infection in a horse n07 506

Mycobacterium avium infection in a HorseN07-506

Ian Hawkins

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


N07 506 history
N07-506 - History

  • An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis

  • Improved on banamine and lidocaine until 8/25/07.

  • Abdominocentesis revealed elevated WBC count and TP

  • Surgery performed on 8/26/07 revealed multiple jejunal and ileal masses and necrotic section of jejunum

  • Due to poor prognosis, the patient was euthanized

  • Necropsy was performed on 8/27/07

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Gross findings
Gross Findings

  • Gross Diagnosis:

    • Granulomatous enteritis, chronic, diffuse with multifocal transmural abscess formation, small intestine.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Gross findings1
Gross Findings

  • Gross Diagnoses:

    • Renal papillary necrosis, chronic, multifocal, marked, Kidneys.

    • Mesenteric and colonic lymphadenomegaly.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Microscopic findings small intestine
Microscopic Findings – Small intestine

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Small intestine with acid fast stain
Small Intestine with Acid-Fast Stain

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Kidney and liver
Kidney and Liver

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Mesenteric lymph node
Mesenteric lymph node

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Diagnoses
Diagnoses

  • Final Anatomic Diagnoses:

    • Granulomatous enteritis, chronic, multifocal, severe with abundant intralesional, acid-fast bacterial rods, small intestine.

    • Granulomatous lymphadenitis, chronic, multifocal, moderate, mesenteric lymph node.

    • Granulomatous typhlocolitis, chronic, multifocal, moderate to marked, cecum and colon.

    • Portal hepatitis, granulomatous, chronic, multifocal, moderate to marked, liver.

    • Renal papillary necrosis, chronic, multifocal, marked, kidneys.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Polymerase chain reaction
Polymerase Chain Reaction

  • Paraffin-embedded sections of intestine were submitted to the NVSL in Ames, IA and Claus Buergelt’s lab at the University of Florida – performed by Elliot Williams.

  • Results from both labs were positive for Mycobacterium avium

    • 16sRNA positive

    • IS1311 positive

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


PCR

  • PCR using IS900 based primers for Mycobacterium avium ssp. paratuberculosis.

  • Tissue was positive using J1-J2 IS900 primers (nested primers).

  • Johne’s? or a variant of M. avium ssp. paratuberculosis.

http://www.waterscan.co.yu/thumbnails/Mycobacterium%20avium.jpg

Map

N07

506

Neg

Map

Map nest

Neg Neg

N07506

P90/P91

J1/J2

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Mycobacterium avium in horses
Mycobacterium avium in Horses

  • Infections in horses are uncommon but have been reported

  • Typically occur in younger adult horses and predominantly affects the gastrointestinal tract; also the lungs and liver with less frequency

  • Clinically horses tend to have chronic weight loss, diarrhea, and hypoproteinemia.

  • M. avium has been isolated from horses with Equine Granulomatous Enteritis, a sporadic idiopathic disease of young horses with similar clinical signs and lesions.

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Mycobacterium infections in horses
Mycobacterium infections in horses

  • Gross findings:

    • Multifocal soft to firm transmural nodules throughout affected regions of the GI tract

    • Ulcerated mucosa

  • Histopathology:

    • Granulomatous inflammation

    • High numbers of multinucleated giant cells

http://freelief.com/temp/april4dee.jpg

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Mycobacterium infections in horses1
Mycobacterium infections in horses

  • Johne’s Disease in a horse?

    • Horses have been experimentally infected with the organism; sporadic cases have been reported, but were unconfirmed and exceedingly rare.

    • A variant of M. avium ssp. paratuberculosis that horses maybe more susceptible to?

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Acknowlegements
Acknowlegements

  • Barbara Sheppard, DVM, PhD, Dipl. ACVP

  • Elliot Williams

  • Giles R.G. Monif

  • John Roberts, DVM, Dipl. ACVP

  • David Taylor, BVSc, Dipl. ACVP

  • Fellow UF Pathology Residents

  • The UFVMC Histology lab staff

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


Questions
Questions?

Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only


ad