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Neuron cell under strong laser beam

Neuron cell under strong laser beam. Living soft matter ?. 江宏仁 2003.07.07. NEURONAL MORPHOGENESIS AND STRUCTURAL PLASTICITY: Axon Initiation Axon Growth Axon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid Motility

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Neuron cell under strong laser beam

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  1. Neuron cell under strong laser beam Living soft matter ? 江宏仁 2003.07.07

  2. NEURONAL MORPHOGENESIS AND STRUCTURAL PLASTICITY: Axon Initiation Axon Growth Axon Guidance Axon Branching Dendritic Growth, Guidance, and Branching Dendritic Spine Formation, Stability, and Rapid Motility Synapse Formation and Stability Axon and Dendrite Retraction

  3. PHENOMENOLOGY

  4. Growrh cone

  5. 4 processes coordinated: protrusion anchorage forward movement tail retraction

  6. Axon Guidance

  7. Guiding neuronal growth with light ? Laser:1064nm 700mW source 50 mW after nosepiece

  8. Theory 1 Optical forces can impact diffusive behavior.

  9. Theory 2 Mechanical manipulation of a membrane.

  10. Theory 3 Dynamic Excitations in Membranes Induced by Optical Tweezers Colloidal effects

  11. PEARLING OF CYLINDRICAL VESICLES Laser-induced rounding

  12. Tests for each theory Theory 1 :* in vitro actin polymeried under laser beam * Listeria movement as membrance moving model Theory 2: *using flow cell to apply shear force on membrance Theory 3: *using indirect phenomena ex: pearling, rounding, retrcation, or in water *direct observe by EM or light scattering

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