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THE MICROSCOPE

THE MICROSCOPE. Types of Microscopes. Light Electron Transmission Scanning. Light Microscope. Uses ordinary white light to view organisms. Electron Microscope. Uses a beam of electrons to view subject 1000 x more powerful than a light microscope Used mostly by universities and labs

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THE MICROSCOPE

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  1. THE MICROSCOPE

  2. Types of Microscopes • Light • Electron • Transmission • Scanning

  3. Light Microscope • Uses ordinary white light to view organisms

  4. Electron Microscope • Uses a beam of electrons to view subject • 1000 x more powerful than a light microscope • Used mostly by universities and labs • EXPENSIVE

  5. Transmission • Transmit electron through the sample • TEM

  6. Scanning • Electrons move across the surface of the sample • SEM

  7. Magnification • Ability to make an object larger

  8. Resolution • Ability to distinguish between 2 small or close objects

  9. Field of View • As you increase magnification you decrease field of view**

  10. Microscope Diagram

  11. Eyepiece-Magnifies (10x) BBody Tube

  12. Eyepiece-Magnifies (10x) BBody Tube Revolving Nose Piece Allows change of objective

  13. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective

  14. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Objectives: increase size of object; Short low power 10x Long high power 40x

  15. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Objectives: increase size of object; Short low power 10x Long high power 40x Diaphragm: controls amount of light

  16. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Objectives: increase size of object; Short low power 10x Long high power 40x Diaphragm: controls amount of light Stage: area on which slide rests

  17. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Objectives: increase size of object; Short low power 10x Long high power 40x Diaphragm: controls amount of light Stage: area on which slide rests Mirror or Light Source: sends light thru the slide

  18. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Objectives: increase size of object; Short low power 10x Long high power 40x Diaphragm: controls amount of light Stage: area on which slide rests Mirror or Light Source: sends light thru the slide Base: support structure for scope

  19. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Arm: offers support to scope Objectives: increase size of object; Short low power 10x Long high power 40x Diaphragm: controls amount of light Stage: area on which slide rests Mirror or Light Source: sends light thru the slide Base: support structure for scope

  20. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Arm: offers support to scope Objectives: increase size of object; Short low power 10x Long high power 40x Stage Clip: holds slide in place Diaphragm: controls amount of light Stage: area on which slide rests Mirror or Light Source: sends light thru the slide Base: support structure for scope

  21. Eyepiece-Magnifies (10x) BBody Tube Coarse Adjustment Moves objectives quickly Revolving Nose Piece Allows change of objective Arm: offers support to scope Objectives: increase size of object; Short low power 10x Long high power 40x Stage Clip: holds slide in place Diaphragm: controls amount of light Stage: area on which slide rests Fine Adjustment: moves objective slowly Mirror or Light Source: sends light thru the slide Base: support structure for scope

  22. How to Carry a Microscope • One hand holding the arm • One hand under the base • Must follow these rules to prevent accidents

  23. Types of Cells • Prokaryotic: no nucleus • Eukaryotic: true nucleus • Only prokaryotic group is bacteria • Eukaryotic groups are fungi, protists, plants and animals

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