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The Microscope Sixth Grade Science Class DTresten.Hildebrandt.2001/2 Modified by TCrannie 02/03 Modified by SDillon 12/03/2007 This is the microscope that is similar to the one we will use in our science lab. It’s the one used in science labs all over the world. Photo by D. Tresten, 2002

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the microscope

The Microscope

Sixth Grade Science Class

DTresten.Hildebrandt.2001/2

Modified by TCrannie 02/03

Modified by SDillon 12/03/2007

slide2

This is the microscope that is similar to the one we will use in our science lab. It’s the one used in science labs all over the world.

Photo by D. Tresten, 2002

background information
Background Information

Two types of lenses:

  • Convex Lens
  • Concave Lens

Molecular Expressions: Light and Color - The Bi-Convex Lens: Interactive Java Tutorial

Molecular Expressions Microscopy Primer: Light and Color - Human Vision: Interactive Java Tutorial

Molecular Expressions: Light and Color - Bi-Concave Lenses: Interactive Java Tutorial

the human eye
The Human Eye

http://micro.magnet.fsu.edu/primer/anatomy/introduction.html

Molecular Expressions Microscopy Primer: Light and Color - Human Vision: Interactive Java Tutorial

microscopes work on the principal of magnification
Microscopes Work on the Principal of Magnification
  • Looking through crystals and raindrops taught people about magnification.
  • Microscopes contain convex lenses, which magnify objects.

Molecular Expressions:

Light and Color –

The Bi-Convex Lens:

Interactive Java Tutorial

table of contents

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

history of the microscope

Back to

Contents

Next Page

History of the Microscope

Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Introduction

General History

A-Z Microscope Welcomes You!

Leeuvenhoek

Famous People

Hooke

anton von leeuvenhoek
Anton Von Leeuvenhoek
  • Lived during 17th century
  • First man to make and use microscope
  • His microscope magnified 270x and looked like this:

A-Z Microscope Welcomes You!

http://micro.magnet.fsu.edu/primer/anatomy/introduction.html

Back to

History

Scroll down this site to see a detailed drawing of the scope:

A-Z Microscope Welcomes You!

Glass-sphere microscope

robert hooke
Robert Hooke

A-Z Microscope Welcomes You!

Hooke wanted to know what something living looked like when it was strongly magnified. Since he knew that he would need to cut a really thin layer to view through his microscope, he chose to view a piece of easily-sliced cork tree…

Next

Page

slide11

http://www.britannica.com/eb/art?id=10558&type=A

Next

Page

Cork oak (Quercus suber) with sections of cork removedEric G. Carle—Shostal/EB Inc.

 ENCYCLOPÆDIA BRITANNICA

cork cells

Credit: National Library of Medicine, NIH

Cork Cells

When Hooke viewed the thin layer of cork through his micro-scope,

this is what he saw:

Next

Page

NIGMS -- Inside the Cell/ Drawing of cork tissue

slide13

The small structures that “made up” cork reminded Hooke of the prison cells of his century…

Cork Cells

Prison Cells

Credit: National Library of Medicine, NIH

NIGMS -- Inside the Cell/ Drawing of cork tissue

Next

Page

slide14

…so he named the small structures “cells.”

Plant Cells

Plant -- Media -- Encarta ® Online

Richard Kirby, Oxford Scientific Films

Plant Cell (viewed through a modern microscope)

Table

of

Contents

Prison Cells

table of contents15

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

why use a microscope to see this

Next Page

Why Use a Microscope?To see this…

Click on Image Gallery

to see why:

CELLS alive!

CELLS alive!

and this

Next Page

And this…

Click on Image Gallery

to see why:

CELLS alive!

CELLS alive!

Dangerous water-born parasite. Most dangerous for persons with

Compromised immune systems.

and this18

Next Page

And this…

Click on Image Gallery

to see why:

CELLS alive!

CELLS alive!

Ascorbic Acid (vitamin C) crystals.

and this19

Next Page

And this…

Click on Image Gallery

to see why:

CELLS alive!

CELLS alive!

Daphnia (water flea) found in pond water.

and this20

Next Page

And this…
  • Dust mite,
  • found in beds,
  • carpets, and
  • sofas.
  • Eat dead skin
  • cells and
  • crumbs from
  • crackers or
  • cookies.
  • May cause
  • itching if too
  • many of them.

CELLS alive!

CELLS alive!

images seen through microscopes

Back to

Contents

Jump to Cells PPT

Images Seen Through Microscopes

Guess It!

The Molecular Expressions Photo Gallery

Click on Image Gallery to view cells through compound scope

http://www.mos.org/sln/SEM/gallery.html

CELLS alive!

http://www.mos.org/sln/SEM/

Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tutorials - Virtual Scanning Electron Microscopy

table of contents23

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

slide24
Let’s learn the names of the parts of the microscope, so that we can later learn how to use each part…

Click here

to begin

slide25

1

2

3

7

4

8

13

5

12

6

11

Eyepiece (or Ocular) (10x)

See the Names of the Parts

Body tube

Click to see names

Coarse Adjustment Knob

Revolving Nosepiece

Low Power Objective (10x)

4

Arm

High Power Objective (50x)

Fine Adjustment Knob

9

Disc Diaphragm

In-base illuminator Light Source (Mirror, not lamp)

10

Inclination Joint

Base (Legs)

http://www3.telus.net/micron/microscope/lab scope.jpg

Stage

Stage Opening

Stage clips

watch how light travels through a microscope

Back To Table of Contents

Watch How Light Travels Through a Microscope

Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Transmitted Light Microscopy Optical Pathways: Interactive Java Tutorial

table of contents27

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

slide28

Back to

Contents

Repeat this Slide

Magnify 10x

Learn the Functions

Support lenses

A-Z Microscope Welcomes You!

Focus quickly but coarsely

To turn objectives into place over specimen

Hold slide

Hold body tube/Carry

Magnify 10x

Magnify 50X

Slowly and finely move objectives toward/away from specimen to focus finely

Allow more/less light

Send light up through specimen

Bend eyepiece toward your eye

Support microscope

Allow light to reach specimen

to play name my functions in order out loud click on each part to see if you were correct

Back to

Contents

Name My Functions Game

Magnify 10x

To play: Name my functions in order, out loud. Click on each part to see if you were correct.

Support lenses

Focus quickly but coarsely

To turn objectives into place over specimen

Hold slide

Magnify 10x

Hold body tube/Carry

Magnify 50X

Slowly and finely move objectives toward/away from specimen to focus finely

Allow more/less light

Send light up through specimen

Bend eyepiece toward your eye

Support microscope

Allow light to reach specimen

slide30

Back To Table of Contents

Zeiss Microscope(Ours)Name its parts

table of contents31

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

care of the microscope page 1 of 3 click in each blank after you say the answer aloud
Care of the Microscope,Page 1 of 3Click in each blank after you say the answer aloud:

Always carry a microscope with _______ hands, one on the ______ and the other on the ________.

two

arm

base

Click here to view answers

Go to Page 2: Care of Microscope

Back to

Table of Contents

care of the microscope page 2 of 3
Care of the MicroscopePage 2 of 3

Clean lenses with ______ _________ only.

lens

paper

NEVER touch _________ with finger tips.

lenses

Click here to view answers

Go to Page 3: Care of Microscope

Back to

Table of Contents

care of the microscope page 3 of 3
Care of the MicroscopePage 3 of 3

Always keep the scope _____

from the _______ of the

_________.

away

edge

Click here to view answers

table

Go to Page 1: Care of Microscope

Back to

Table of Contents

how to use the microscope

How to Wake It Up

How to Focus

How to Put it to Bed

How to Make a Wet Mount

Back To Table of Contents

How to Use the Microscope

use of the microscope page 1

Back to

Contents

More About Use

To Use the Scope, Wake It Up:

Use of the MicroscopePage 1
  • Check: Is objective AWAY from stage?
  • Place LOW powered (10x) objective over stage opening.
  • Plug in scope.
  • Place wet-mounted slide on stage (only if objective is far away).
  • Turn on lamp.

Click to see

5 steps

how to use the microscope37

How to Wake It Up

How to Focus

How to Put it to Bed

How to Make a Wet Mount

Back To Table of Contents

How to Use the Microscope

what does focus mean

Back to

Contents

What does “focus” mean?

In this site, raise and lower the “focus” knob to see how blurry and sharp the image of the specimen will appear.

Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tutorials - Virtual Scanning Electron Microscopy

How to

Focus

slide39

Back to

Contents

Step 1: Focusing

How to Focus on a Specimen:

Click below to practice focusing:

Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tutorials - Virtual Scanning Electron Microscopy

set disc diaphragm to 1 least light setting

Back to

Contents

Set Disc Diaphragm to 1,“Least Light” Setting

See Close-Up of

Disc Diaphragm

See

Next Step

slide41

Close-up of disc diaphragm

(as seen from underneath

the stage)

Back to

Contents

See

Next Step

set scope to low power 100x
Set Scope to Low Power (100x)

In other words, set the 10x objective in place over the stage opening.

slide43

Back to

Contents

Place specimen (which is on a slide) directly over the stage opening and secure it with stage clips…

See

Next Step

slide44

Back to

Contents

Looking from the side, use the coarse adjustment knob to raise the stageas close as possible to the objectives…

See

Next Step

slide45

Back to

Contents

Looking through the eyepiece for the first time (with both eyes open), use the coarse and/or fine adjustment knob/s untilthe specimenis in sharp,clearfocus.

See

Next Step

use of the microscope page 3

Back to

Contents

More About Use

How to Focus on a Specimen, Cont’d:

Use of the MicroscopePage 3

If there is too little light, rotate the discdiaphragm to another opening.

See

Next Step

Click below to practice focusing:

Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tutorials - Virtual Scanning Electron Microscopy

slide47

Back to

Contents

Note: If the object is a blur or just a shadow, it is NOT in focus. Keep turning the coarse/fine adjustment knobs.

See

Next Step

slide48

Back to

Contents

How to Focus on a Specimen, Cont’d:

Click below to practice focusing:

Molecular Expressions Microscopy Primer: Electron Microscopy Interactive Java Tutorials - Virtual Scanning Electron Microscopy

how to use the microscope50

How to Wake It Up

How to Focus

How to Put it to Bed

How to Make a Wet Mount

Back To Table of Contents

How to Use the Microscope

use of the microscope page 4

Back to

Contents

More About Use

Always put our microscopes “to bed” after labs:

Use of the MicroscopePage 4
  • Turn off the lamp
  • Unplug scope
  • Place 10x (LOW powered) objective over stage opening
  • Move stage away from objectives
  • Take out slide
  • Clean stage, slide and cover slip

Click to view 6 steps

for putting micro-

scope to bed

how to use the microscope52

How to Wake It Up

How to Focus

How to Put it to Bed

How to Make a Wet Mount

Back To Table of Contents

How to Use the Microscope

how to prepare a slide how to make a wet mount

Back to

Contents

More About Use

How to Prepare a Slide (How to Make a Wet Mount)

Microscope Lab - Wet Mounts

Staining Cells

table of contents54

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

types of microscopes

Back to

Contents

See real images!

Types of Microscopes

Zeiss

Microscope

(Ours)

Compound Light Microscope

Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Introduction

Electron Microscope

http://www.mos.org/sln/SEM/

Molecular Expressions Microscopy Primer: Museum of Microscopy - Siemens Elmiskop IA Transmission Electron Microscope

slide56

Back To Table of Contents

This is the microscope that we will use in our science lab. It’s the Zeiss model used in science labs all over the world.

http://micro.magnet.fsu.edu/primer/anatomy/introduction.html

table of contents57

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

other topics

Back to

Contents

Other Topics

How to Safely Collect Pond Water Samples

Microscopic Life in Water

Cool Links about Microscopy

Glass-sphere microscope

http://www.uq.edu.au/nanoworld/images_1.html

Ethical Use of the Microscope

Setting Up a Microscope Lab

table of contents59

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

permissions photographs

Back To Table of Contents

Permissions: Photographs

Photographs taken by D. Tresten, 2002, may be reused by teachers and students in an educational setting.

http://micro.magnet.fsu.edu/primer/anatomy/introduction.html

Encyclopedia Britannica

(Photograph of Cork tree):

You may print or download Materials on the Site for your personal, non-commercial use, provided you keep intact all copyright and other proprietary notices.

Content on the CELLS alive! website is the property of Quill Graphics and is subject to international copyright laws. The site is provided as a free classroom educational resource to thousands of students and teachers each day and we encourage the use of the site and images in the classroom setting. However, any use outside the school classroom is restricted.

  • STUDENTS AND TEACHERS ARE GRANTED PERMISSION TO:
    • use images from this site in class reports, projects, homework, and lectures, including printing in handouts and classroom projection using presentation software like PowerPoint®
    • post three images on a SCHOOL WEBSITE if you provide both a credit and a link to CELLS alive!
permissions clip art

Back To Table of Contents

Permissions: Clip Art

Clip Art – From Microsoft Design Gallery

permissions images

Back To Table of Contents

Permissions: Images

A-Z Microscope Welcomes You!

Pending – E-mail sent 5/8/02

© 1997-2002 A-Z Microscope Corporation.  All rights reserved.Reproduction in whole or in part in any form or medium without express written permission of A-Z Microscope Company is strictly prohibited.

If given permission, I promised to include live link over image

permission images from molecular expressions

Back To Table of Contents

Permission: Images from Molecular Expressions

PERMISSION FOR PHOTOS FROM THIS

SITE PENDING – sent E-mail 5/8/02

Molecular Expressions Microscopy Primer: Museum of Microscopy

Molecular Expressions Microscopy Primer: Virtual Microscopy: Interactive Java Tutorials

Molecular Expressions Microscopy Primer: Anatomy of the Microscope - Microscope Objectives

http://micro.magnet.fsu.edu/primer/anatomy/introduction.html

table of contents64

Use

  • Parts
  • Types
  • History
  • Care
  • Images

Table of Contents

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