1 / 14

Protein delivery: DNA nanostructures and cell-surface targeting

Protein delivery: DNA nanostructures and cell-surface targeting. Harvard iGEM August 27, 2006. The Machine. Goal: Future modular drug delivery. target cell. drug. Molecular containers in nature. Hard to duplicate artificially. http://www.biology4kids.com/files/art/cell_over1.gif.

galya
Download Presentation

Protein delivery: DNA nanostructures and cell-surface targeting

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Protein delivery:DNA nanostructures and cell-surface targeting Harvard iGEM August 27, 2006

  2. The Machine • Goal: Future modular drug delivery target cell drug

  3. Molecular containers in nature • Hard to duplicate artificially http://www.biology4kids.com/files/art/cell_over1.gif http://micro.magnet.fsu.edu/cells/viruses/images/virus.jpg

  4. DNA Nanostructures Overview • DNA can be used to approximate arbitrary 3D structures Ned Seeman, NYU Paul Rothemund, Caltech William Shih, Harvard WILLIAM M. SHIH, JOEL D. QUISPE & GERALDF. JOYCE Nature427, 618ﾐ621 (2004); http://www.dna.caltech.edu/~pwkr/ http://seemanlab4.chem.nyu.edu/nano-cube.html

  5. Motivation: Why DNA? • The power of DNA • Nanometer scale • Covalent modifications possible • Inexpensive synthesis • Highly programmable/designable

  6. Design Details: Scaffolded Oragami M13 viral genome 7308 bases long Add ~180 helper strands in Mg++ buffer Heat to near boiling. (90 C)

  7. Design Details: Scaffolded Oragami

  8. Design Details: Scaffolded Oragami When the sample reaches room temperature (2hrs later), the origami have folded http://www.dna.caltech.edu/~pwkr/

  9. Design Details: Positional Control

  10. Design Details Double-ply barrel and lid Lid: 33 nm across, 28 nm long Barrel: 27.5 nm long, 27.6 nm across

  11. Exciting EM Images

  12. Exciting EM Images

  13. To be continued • Can a protein be protected from protease if attached inside the box? • Lid attachment • Lid removal protease protein protease protein

  14. Acknowledgements • Harvard TFs -Shawn Douglas, Nick Stroustrup, Chris Doucette • Harvard advisers- Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal • iGEM ambassadors • iGEM directors

More Related