Staining differential stains
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Staining: Differential Stains. Gram stain. Acid-fast stain. Gram positive. Gram negative. Outer membrane. Peptidoglycan. Plasma membrane. Gram negative. Gram positive. Gram Stain. before staining primary stain, mordant decolorizer counterstain. ?. Known Gram +. Known Gram -.

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Staining: Differential Stains

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Staining: Differential Stains

Gram stain

Acid-fast stain


Gram positive

Gram negative

Outer membrane

Peptidoglycan

Plasma membrane


Gram negative

Gram positive

Gram Stain

  • before staining

  • primary stain, mordant

  • decolorizer

  • counterstain


?

Known Gram +

Known Gram -

Unknown

When staining an unknown, add controls!


Acid-fast Stain

mycobacteria

medlib.med.utah.edu/WebPath/ TUTORIAL/MTB/MTB013.html


http://www.chemsoc.org/chembytes/ezine/images/1998/evans_fig2.jpg

www.bio.gasou.edu/Bio-home/ Pung/pungbiol3240urls.html

Acid-fast Bacterial Cell Wall


Acid-fast technique

acid-fastspecies

non acid-fast species

1. carbolfuschin

2. acid-alcohol

3. methylene blue


Gramstainprotocol


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