Microarray (Gene Chip) Technology
Download
1 / 73

Microarray (Gene Chip) Technology - PowerPoint PPT Presentation


  • 117 Views
  • Uploaded on

Microarray (Gene Chip) Technology. PART 1. Part 2. Part 3. Part 4. Features – Parallelism • Thousands of genes simultaneously – Miniaturization • Small chip size – Multiplexing • Multiple samples at the same time – Automation • Chip manufacturing • Reagents. Part 1.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Microarray (Gene Chip) Technology' - amelia-hurley


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

PART 1

Part 2

Part 3

Part 4


Features

– Parallelism

• Thousands of genes simultaneously

– Miniaturization

• Small chip size

– Multiplexing

• Multiple samples at the same time

– Automation

• Chip manufacturing

• Reagents


Part 1

PART 2

Part 3

Part 4


Step

Step

Step


Step 2

Step 1

Step 3

Step 2

Step 3


Microarray Fabrication

  • Fabrication via Printing (cDNA chip)

    • DNA sequence stuck to glass substrate

    • DNA solution pre-synthesized in the lab

  • Fabrication In Situ(Affymetrix chip)

    • Sequence “built”

    • Photolithographic techniques use light to release capping chemicals

    • 365 nm light allows 20-m resolution


Fabrication via printing

cDNA chip

Fabrication In Situ: Affymetrix chip



Microarray Features

– Parallelism

• Thousands of genes simultaneously

– Miniaturization

• Small chip size

– Multiplexing

• Multiple samples at the same time

– Automation

• Chip manufacturing

• Reagents





Fabrication via printing

cDNA chip

Fabrication In Situ: Affymetrix chip



Poly-A

mRNA

10% Biotin-labeled Uracil

Antisense cRNA

cDNA

IVT

AAAA

Cells

L

L

L

L

L

L

Fragment (heat, Mg2+)

Labeled

fragments

Hybridize

Wash/stain

Scan

Affymetrix microarray process


Hybridization and staining

L

L

L

L

L

L

L

L

L

L

L

L

Hybridization and Staining

Biotin

Labeled cRNA

GeneChip

Hybridized Array

+

+

SAPE

Streptavidin-

phycoerythrin


The result
The Result

  • A light source scans the array, causing the dyes to fluoresce

  • The glow is picked up by a sensor and is used to determine the relative abundance of the RNA

  • This information must be processed to determine the level of activity for each expressed gene





Part 1

Part 2

Part 3

PART 4



ad