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. b - Carotene . . Vitamin A and b - Carotene Determination. b-CAROTENE STANDARD ABSORBANCES AT 440 AND 620 nm . . . . . . . . . . . . . . . . . . . . . . A at 440 nm . and 620 nm. at 440 nm. at 620 nm. A. A. Use absorbances at 440 nm and then convert this to absorbance at 620 nm and subtract from the absorbance at 620 nm to determine the absorbance at 620 due to Vitamin A. .

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