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Solution for Bile Acid Analysis

Bile acids are synthesized from cholesterol in the liver hepatocytes. The conversion of cholesterols into bile acids involves various enzymes in modifying cholesterol steroid cores, removing sidechains, and conjugating glycine and taurine to form primary bile acids. Many microorganisms in the gut can further metabolize bile acids to produce secondary bile acids.<br>https://www.creative-proteomics.com/services/bile-acids-analysis-service.htm<br>

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Solution for Bile Acid Analysis

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  1. SOLUTION FOR BILE ACID ANALYSIS Why bile acid analysis? Bile acids are synthesized from choles- terol in the liver hepatocytes. The con- version of cholesterols into bile acids involved various enzymes in modifying cholesterol steroid cores, removing side chains, and conjuga�ng glycine and taurine to form primary bile acids. A large number of microorganisms in the gut can further metabolize bile acids to produce secondary bile acids. Changes in the type and con- tent of bile acids can affect the host microbiota distribu�on, intes�nal barrier func�on, signal transmission, and other biological processes. What We Do U�lizing the UPLC-MS/MS pla�orm, Crea�ve Proteomics can well separate a variety of bile acid isomers and isotopically label them as internal standards, thus accurately quan�-fying 70+ bile acids in various biological samples for different detec�on needs. Service Metabolic Pathway Sample Requirement Tissue: ≥ 10 mg Cell: ≥ 2×106 Blood/plasma/serum: ≥ 20 µL Urine: ≥ 10 µL Feces: ≥ 5 mg DBS: ≥ 20 µL Bile acid synthesis and metabolism Bile Acid Analysis Gut co-metabolism -Synthesis and Metabolism Assay Cholesterol metabolism Blood-brain barrier Tissue: ≥ 10 mg Cell: ≥ 2×106 Blood/plasma/serum: ≥ 50 µL Urine: ≥ 50 µL Bile acid synthesis Bile Acid Precursor Analysis Blood-brain barrier Cholesterol metabolism Bile Acids (Biocrates Bile Acids Kit) 50 µL 30-40 mg Bile acid metabolism Advantages Absolute Quan�ta�on High Sensi�vity Using external standards for quan�fica�on and internal stan- dards for correc�on, delivering more accurate qualita�ve and quan�ta�ve analyses. The UPLC-MS/MS pla�orm relies on the mul�ple reac�on moni- toring (MRM) mode of triple quadrupole (QQQ), capable of detec�ng samples as low as the pg level. Contact Us © Creative Proteomics All Rights Reserved.

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