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“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA”

“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA”. Angola:  ~ 14 million inhabitants. low HIV/AIDS prevalence levels (~ 2.1%, UNAIDS 2008 ). HIV-1 within the Angolan population shows very complex and high genotype diversity.

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“HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA”

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  1. “HIV-1 GENETIC VARIABILITY AND INTERSUBTYPE RECOMBINANT FORMS CIRCULATING IN THE NORTH OF ANGOLA” Angola:  ~ 14 million inhabitants • low HIV/AIDS prevalence levels (~ 2.1%, UNAIDS 2008). • HIV-1 within the Angolan population shows very complex and high genotype diversity.  may cause unprecedented challenges to diagnostic, treatment and prevention of HIV-1. The aim of this work was to investigate the genetic diversity of HIV-1-positive Angolan samples.

  2. METHODS and RESULTS: • n = 96 HIV-1-positive Angolan patients were diagnosed and collected between 2008 and 2010 in Bengo. • Samples were genotyped based on phylogenetic and recombination analysis from pol (PR/RT) gene. • High HIV-1 genetic diversity was depicted in the pol gene, with a predominance of intersubtype recombinant forms, CRFs and URFs (each with 21%), following subtypes C and F1 (each with 15%), U (untypable sequences, 7%), G (6%), D and H (each with 5%), A (4%) and K (each 1,04%) were found.

  3. METHODS and RESULTS: • Nine out of twenty (45%) CRFs samples formed a unique cluster (U/H pattern) in the PR/RT region and were selected for DNA sequencing of the gag and partial env (gp41) gene regions. . Further analysis of the gag and env regions indicated a CRF06_cpx gag/ H env gp41 pattern for all nine samples. Gag (758 1905 bp): CRF06_cpx env (7638 8250 bp): H

  4. CONCLUSIONS: ACKNOWLEDGEMENTS: • Molecular data have indicated a complex degree of HIV-1 group M genetic variability within the Angolan samples. • Combined analysis of the nine sequences presenting the same recombinant profile in PR/RT- gag- env gp41 gene regions suggest a potential new HIV-1 CRF circulating in the North of Angola. • LABORATORY OF CLINICAL ANALYSIS: • Ms. Maria de Lurdes • Ms. Judith • Ms. Ludovina PhD Scholarship • LABORATORY OF AIDS & MOLECULAR IMMUNOLOGY: • Drª. Mariza Morgado • Dr. Gonzalo Bello • Drª. Monick Guimarães • CENTRO DE SAÚDE SÃO LUCAS: • Drª. Martha Sojka • Patients

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