1 / 8

Amine-presenting electro-conducting scaffolds

Amine-presenting electro-conducting scaffolds. Jae Young Lee and Christine E. Schmidt. Motivation. Amino-polypyyrole (APPy) [Issue] Neurons normally adhere poorly to regular PPy films

zuwena
Download Presentation

Amine-presenting electro-conducting scaffolds

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Amine-presenting electro-conducting scaffolds Jae Young Lee and Christine E. Schmidt

  2. Motivation • Amino-polypyyrole (APPy) [Issue] • Neurons normally adhere poorly to regular PPy films • Conventional adhesion methods involves the external treatment of ‘ECM molecules (laminine, fibronectin, collagen etc’ and ‘ECM fragments (RGD, IKVAV etc)’, or ‘polycationic polymers (Polylysine, PEI, PAA)’. [Beneficial aspects] • APPy has positive charge – possible increase in cell adhesion. • Theoretically permanent display of positive charge on the substrate. • Has potential to be used for interfactial materials between neurons and electrodes (neural probes, sensors, and tissue engineering scaffolds)

  3. Synthesis 1-aminopropyl pyrrole was synthesized from 1-cyanoethyl pyrrole using a reduction reaction with LiAlH4. The reflux reaction was carried out for 20 hours in a round bottom flask in ethyl ether under Ar gas. Purity was confirmed using NMR and TLC (ninhydrin). [1] 1-(2-cyanoethyl)pyrrole [2] 1-(3-aminopropyl)pyrrole

  4. Water Contact Angle • Water contact angle was then measured to see how hydrophibic or hydrophillic the samples were: • Hydrophobic: Poor cell adhesion and destruction of protein activity. • Hydrophillic: charged on sample. Is desired.

  5. Material characterization • Conductivity: 4 pt probe techniques employed • Surface amine: quantified using ninhydrin methods

  6. Cell Adhesion Studies (MTS): Fibroblast

  7. A B C Cell Adhesion Studies: FL images Live staining of fibroblast cultured on the sample: (A) reg. PPy, (B) APPy(50:50), and (C) APPy(100).

  8. Acknowledgement Jae Young Lee Dr. Christine Schmidt Derek Ensign Gregory Abraham

More Related