1 / 15

Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices. Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in

winter
Download Presentation

Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Localized and automated chemical and oxygen delivery system for microfluidic brain slice devices Author: Gene Yu Co-Authors: Dr. AlexBlake Dr. David Eddington July 29, 2010 NSF Research Experiences for Undergraduates (REU) in Novel Advanced Materials and Processing with Applications in Biomedical, Electrical and Chemical Engineering

  2. Objective • Design localized and automated delivery system for microfluidic brain slice devices (μBSDs) • Delivers chemicals/drugs and gases • Automatically mixes oxygen • Characterize delivery of chemicals through tissue Image taken from Mohammed, J, Caicedo, H, Fall, C, & Eddington, D. (2008). Microfluidic add-on for standard electrophysiology chambers. Lab Chip, 8, 1048-1055.

  3. Motivation • μBSDs allow in vitro study of: • Neurogenesis • Synaptogenesis • Regeneration • Protein expression • Responses to physical trauma Image taken from http://www.koki.hu/cdnb/research/research.html Rambani, K, Vukasinovic, J, Glezer, A, & Potter, S. (2009). Culturing thick brain slices: an interstitial 3d microperfusion system for enhanced viability. Journal of Neuroscience Methods, 180, 243-254.

  4. Relevance • Chemical Delivery • Spatial resolution • Current systems affect entire slice with drug • Desire stimulation of specific areas • Oxygen Delivery • Automatic Mixing • Saves money • Pre-mixing is not required • Automation • Multi-tasking Image modified fromhttp://www.imagingeconomics.com/issues/articles/MI_2006-08_01.asp

  5. Oxygen Mixing and DeliveryExperimental Set-Up • Oxygen concentration measured by a NeoFox Fiber Optic Oxygen (FOXY) sensor Valves Gas Feed Lines: 0% and 21% Y Connector Output Tube

  6. Oxygen Mixing and DeliveryOutput

  7. Oxygen Mixing and DeliveryGraphical User Interface (GUI)

  8. Delivery CharacterizationμBSD Design Reservoir T Channel Delivery Channel

  9. Delivery CharacterizationExperimental Set-up DI Water Lines T Channel Valve μBSD Vacuum Line

  10. Delivery CharacterizationNo Tissue Delivery 10ms 15ms 20ms 25ms 2x5ms 3x5ms 4x5ms 5x5ms

  11. Delivery CharacterizationLinear Relationships: No Tissue 860 Width (µm) 1320 Width (µm)

  12. Delivery CharacterizationLinear Relationships: With Tissue Full Dose Chemical Delivery Maximum Intensities Intensity Intensity 4 2 0 2 6 Number of 5ms Pulses Time (min) Pulsing Dose Chemical Delivery Maximum Intensities Intensity Intensity 30 20 2 0 10 Time (min) Valve Open Time (ms)

  13. Delivery CharacterizationGUI

  14. Conclusions • Oxygen Delivery • Precise and accurate results • Reliable oxygen switching • Chemical Delivery • Demonstrated linear relationship • Area of effect: ~2mm x 2mm • Selective and independent valve control

  15. Acknowledgements • Funding • NSF-REU • DoD-ASSURE • EEC-NSF Grant # 0755115 • REU Directors • Christos G. Takoudis, Ph.D. • Greg Jursich, Ph.D. • Research Advisor • David Thomas Eddington, Ph.D. • Mentors • Alexander Blake, Ph.D. • Gerardo Mauleon

More Related