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Breast Cancer ECM Visualization using Multiphoton Imaging

Breast Cancer ECM Visualization using Multiphoton Imaging. Jeremy Bredfeldt LOCI, Morgridge Institute for Research. H&E and SHG. Protocol Thaw & fix sample Section sample to 200 microns on a vibratome Image with SHG Paraffin embed tissue wafer Section and stain for H&E Slide scan

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Breast Cancer ECM Visualization using Multiphoton Imaging

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  1. Breast Cancer ECM Visualization using Multiphoton Imaging Jeremy Bredfeldt LOCI, Morgridge Institute for Research

  2. H&E and SHG • Protocol • Thaw & fix sample • Section sample to 200 microns on a vibratome • Image with SHG • Paraffin embed tissue wafer • Section and stain for H&E • Slide scan • Green = SHG signal (Collagen Type I)

  3. Normal Associated Tissue H&E SHG 11.6 mm

  4. Invasive Ductal Carcinoma SHG H&E

  5. Fibroadenoma H&E SHG

  6. Mucinous Carcinoma 7.5 mm H&E SHG

  7. Intrinsic Fluorescence • Green = SHG • Red = Autofluorescence from FAD or Collagen type III

  8. FibroAdenoma

  9. Extrinsic Fluorescence • Green = SHG (Collagen type I) • Red = E-Cadherin stained cells & Autofluorescence from Collagen type III

  10. Terminal Ductal Lobular Unit (Red) Surrounded by Collagen (Green)

  11. Duct in Cross Section, Surrounded by IDC cells IDC Duct

  12. IDC Cells Near a Vessel with ECM IDC Cells Vessel

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