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Optimal Seeding Density for Enhanced CTL Expansion in Cancer Immunotherapy

This study investigates the impact of seeding density on the expansion of EBV-CTLs, crucial for cancer treatment. Supplementary figure details the fold expansion at various densities over weeks and the % of specific lysis at different effector/target ratios. Findings suggest seeding density significantly influences CTL proliferation and lytic activity.

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Optimal Seeding Density for Enhanced CTL Expansion in Cancer Immunotherapy

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  1. Seeding density EBV-CTLs x cm2 Supplementary figure 1 40 40 35 35 30 30 25 25 20 20 15 15 10 10 5 5 0 0 5x10 5 1.2x10 5 3.1x10 4 A B Fold expansion Fold expansion Week 2 Week 2 Week 3 Week 3 Week 4 Week 4 C % of specific lysis 40-1 20-1 10-1 5-1 40-1 20-1 10-1 5-1 40-1 20-1 10-1 5-1 Effectors/target ratio Effectors/target ratio Effectors/target ratio Seeding density EBV-CTLs x cm2 5x105 1.2x105 3.1x104

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