Identification of ibd using an electronic e nose
Download
1 / 21

Identification of IBD using an electronic e-nose - PowerPoint PPT Presentation


  • 112 Views
  • Uploaded on

Identification of IBD using an electronic e-nose. Covington JA 3, , Westinbrink E 3 , Nwokolo C 1 , Bardhan KD 4 , Arasaradnam RP 1,2 1University Hospital Coventry & Warwickshire & 2Clinical Sciences Research Institute, Medical School, University of Warwick

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Identification of IBD using an electronic e-nose' - vevay


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Identification of ibd using an electronic e nose

Identification of IBD using an electronic e-nose

Covington JA3,, Westinbrink E3, Nwokolo C1, Bardhan KD4, Arasaradnam RP1,2

1University Hospital Coventry & Warwickshire &

2Clinical Sciences Research Institute, Medical School,

University of Warwick

3School of Engineering, University of Warwick, 4Rotherham NHS Trust

11th BROAD Meeting, Los Angeles – March 2013

1


Identification of ibd using an electronic e nose

Distal UC; proximal constipation

PL, age 64

11 weeks after PEG: 4 / 20 markers

Before PEG: 13 / 20 markers

Cow 100%

Horse 100%

Cow 50%

Saw dust 50%

--horse-oid!

2


Composite animal volatile organic compounds vocs chromatogram profile gc
Composite animal Volatile Organic Compounds (VOCs) - chromatogram profile (GC)

Pilot – Animal Studies

Chicken = Black

Horse = Pink

Cow = Blue

Time

Quantity: Measured as ppm or calculated as area under curve (AUC)

3


Identification of ibd using an electronic e nose

Sniffing diseases..… chromatogram profile (GC)

Can we smell these chemcical?

(>5 senses)


Smell @ warwick
‘Smell’ @ Warwick chromatogram profile (GC)

Life in ‘Smell’

Persaud & Dodd Nature 1982

First research group dedicated to the sense of smell

First company making artificial olfaction instruments

First commercial products manufactured here…

Long history of smell research…


What can we analyse
What can we analyse? chromatogram profile (GC)

Urine/Faecal/Breath samples from animals & patients

Chemical analysis of odours emanating from the sample – essentially sniffing!

6


Volatile organic compounds vocs
Volatile organic compounds (VOCs) chromatogram profile (GC)

  • Disease alters gut flora - altered fermentation patterns which alters the composition of gases emitted from urine

  • Organic compounds that have high vapour pressure at normal room temperature.

  • Mainly from colonic fermentation by gut bacteria and partly from physiological metabolic processes

  • Released in breath, urine, faeces, blood

  • A potential diagnostic biomarker in IBD


Technologies used
Technologies used chromatogram profile (GC)

Ion Mobility Spectrometry

Electronic nose

GCMS


Study design
Study design chromatogram profile (GC)

62 subjects – 3 groups

Urine collected and analysed using E-nose and FAIMS

Data analysed using Principal Component analysis


Identification of ibd using an electronic e nose

E-nose sensor response chromatogram profile (GC)


Differentiating ibd using e nose
Differentiating IBD using E-nose chromatogram profile (GC)

The maximum response minus the minimum response

is used as a feature for data analysis


Results e nose
Results – E nose chromatogram profile (GC)

Discriminant Function (DF) Analysis of Fox 4000 data


Faims field asymetric ion mobility spectrometry
FAIMS – Field Asymetric Ion Mobility Spectrometry chromatogram profile (GC)

  • Simple fast analysis of vapours and gases

  • Detects chemicals in complex mixtures

  • Identifies by mobility (ion movement through an electric field)

  • Mobility determined by molecule size and mass


Faims data
FAIMS Data chromatogram profile (GC)

  • 4,000 variables from Wavelet transform

  • Cluster Analysis to identify key variables

  • Use 20 for identification

1st 500 variables

Control – Disease state


Study design1
Study design chromatogram profile (GC)

105 subjects – 2 groups

Urine collected and analysed using FAIMS

Data analysed using Principal Component analysis


Results faims n 105
Results – FAIMS (n = 105) chromatogram profile (GC)

  • 40 Crohns

  • 40 Ulcerative Colitis

  • 25 Controls

Each sample re-classified based on remaining samples


Identification of ibd using an electronic e nose

Results – FAIMS flare vs remission chromatogram profile (GC)



Summary of chemical peaks in volunteers and crohn s uc patients
Summary of chemical peaks in volunteers and Crohn’s , UC patients

What are we detecting?

Possible Key chemicals

  • In excess of 20 chemicals modulated;

  • Likely key chemicals:

    • ethyl esters propanoic or butanoic acids, butanoic acid

    • methyl ester, 3-methyl butanoic acid, 1-butanol, 1-propanol and indole

HA=hydrogen azide (HN3); APossible Key chemicalsA=acetic acid (CH3COOH); PG=propylene glycol (C3H6(OH)2); A=aldehydes; K=ketones; OA=organic acids.


Conclusions
Conclusions patients

Clear disease separation between controls, ulcerative colitis and Crohn’s disease

Able to detect between disease flares and remission

VOC correlation with GC

Potential first line diagnostic modality in patients with suspected IBD

Inexpensive novel tool, non invasive and potentially provides point of care diagnosis


Thank you questions

Acknowledgements: patients

Nathalie Ouaret (PhD)

Nabil Quraishi (MSc)

Eic Westinbrink (PhD)

Nicki O’Connel, RGN

Catherine Bailey, RGN

BROAD Foundation

BRET

BDRF

Thank you - Questions