1 / 9

PBIO 427/527: Molecular Genetics Lecture 1 - Review

Discover the key milestones in molecular genetics from DNA as the genetic material to advancements in biotechnology such as recombinant DNA technology and genetic engineering. Learn about crucial discoveries like the identification of genes, DNA structure, and the central dogma of biology. Explore the history of molecular genetics from important events in the early 20th century to recent breakthroughs in DNA sequencing and genetic manipulation.

ver
Download Presentation

PBIO 427/527: Molecular Genetics Lecture 1 - Review

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PBIO 427/527: Molecular GeneticsLecture 1 - Review Molecular Genetics-terminology & milestones DNA is the genetic material DNA structure Central Dogma

  2. Terminology • Biotechnology-production of goods and services using biological organisms, systems, and processes • Recombinant DNA technology-a set of techniques for manipulating DNA, including: the identification and cloning of genes; the study of the expression of cloned genes; and the production of large quantities of gene product • Genetic engineering-the process of transferring DNA from one organism into another that results in a genetic modification • Molecular biotechnology-rDNA technology + biotechnology • Molecular genetics-the study of the inheritance and expression of traits at the molecular level with the most important molecules here being DNA, RNA and protein

  3. Milestones in molecular genetics 1928-Fred Griffith provides evidence for a genetic transforming factor (DNA) 1944-Avery, MacLeod & McCarty determine DNA is the genetic material 1952-Hershey & Chase also determine that DNA is the genetic material 1953-Watson & Crick determine the structure of DNA 1970-First restriction endonuclease isolated 1973-Boyer & Cohen establish recombinant DNA technology 1976-DNA sequencing techniques developed 1980-U.S. Supreme Court rules that genetically modified micro-organisms can be patented 1981-First DNA synthesizers sold 1988-PCR method published 1990-Human genome project initiated 1996-Complete DNA sequence of a eukaryote (yeast) determined 1997-Nuclear cloning of a mammal (a sheep named Dolly) 2000-Arabidopsis genome sequenced 2001-Human genome sequenced 2002-Complete human gene microarrays (gene chips) available

  4. Evidence that DNA is the genetic material (Griffith-1928; Avery, McLeod & McCarty-1944) Heat-killed S extract + DNase + R  Mouse Lives Heat-killed S extract + RNase + R  Mouse Dies Heat-killed S extract + Protease + R  Mouse Dies

  5. Evidence that DNA is the genetic material (Hershey & Chase-1944)

  6. Chemical structure of DNA & RNA

  7. Chemical structure of dsDNA

  8. Chemical structure of dsDNA

  9. Central Dogma of Biology transcription translation $$$ DNA RNA Protein reverse transcription Molecular Biotechnology DNA replication

More Related