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A. 2.8 kb. Sca I. Sca I. Probe. S-1567. laeA. Sca I. Hyg r. pSAK-Dislae01. Sca I. 3.5 kb. 2.3 kb. Sca I. Sca I. Sca I. Sca I. Hyg r. laeA disruptant. B. C. laeA disruptant. Parent. Complement. 1. 2. P. (kb). Top. Dark. 3.5. 2.4. Back. Top. Light. Back.

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  1. A 2.8 kb ScaI ScaI Probe S-1567 laeA ScaI Hygr pSAK-Dislae01 ScaI 3.5 kb 2.3 kb ScaI ScaI ScaI ScaI Hygr laeA disruptant B C laeA disruptant Parent Complement 1 2 P (kb) Top Dark 3.5 2.4 Back Top Light Back laeAdisruptant Fig. S2

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